Abstract
The rate of decomposition of urea in citrated donors' plasma by soluble urease and by urease immobilized by addition to the carboxymethyl ester of cellulose, the 2-(3′-amino-4′-methoxyphenyl) sulfonylethyl ester of cellulose, the diethylaminoethyl ester of cellulose, stained with dichlorotriazine dye, or the grafted copolymer of cellulose and polyglycidylmethacrylate was found to be closely similar. Preparations of immobilized urease can be used repeatedly to decompose urea in citrated donors' plasma. Periodic treatment of these preparations with cysteine solution resulted in a smaller degree of decrease in the enzyme activity of the immobilized urease during repeated use.
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Mel'nik, I.P., Molodenkov, M.N., Mashkov, O.A. et al. Investigation of the possible use of immobilized urease for decomposing urea in blood plasma. Bull Exp Biol Med 83, 494–496 (1977). https://doi.org/10.1007/BF00807486
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DOI: https://doi.org/10.1007/BF00807486