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Effect of presynaptic neurotoxins from bee and cobra venom on spontaneous mediator secretion from mouse motor nerve ending

  • Pathological Physiology and General Pathology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Phosphorylases A2 (PLA) from bee and cobra venoms cause triphasic changes in the frequency of miniature endplate potentials (MEPPs) recorded in the mouse diaphragm: an initial decrease of liberation of mediator followed by an increase, followed in turn by complete blocking of liberation. Removal of Ca++ from the perfusing solution (to concentrations below 10−9M) prevented the presynaptic effect of the two PLAs. If the PLA was washed off with calcium-free solution, subsequent treatment with standard solution (2 mM Ca++) caused an increase in the frequency of MEPPs. Agents increasing the Ca++ concentration in the axoplasm (K+ ions, hypertonic sucrose, and the uncoupling agent 4, 5, 6, 7-tetra-2-trifluoromethylbenzimidazole) caused an increase in the MEPP frequency in muscles poisoned with PLA. Blocking the liberation of mediator evidently was not caused by exhaustion of its reserves.

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Authors
  1. M. A. Kamenskaya
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  2. L. G. Magazanik
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  3. E. R. Kotova
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  4. N. K. Samybaldina
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  5. A. I. Miroshnikov
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  6. U. R. Apsalon
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Kamenskaya, M.A., Magazanik, L.G., Kotova, E.R. et al. Effect of presynaptic neurotoxins from bee and cobra venom on spontaneous mediator secretion from mouse motor nerve ending. Bull Exp Biol Med 87, 402–405 (1979). https://doi.org/10.1007/BF00806665

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  • DOI: https://doi.org/10.1007/BF00806665

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