Abstract
DNA content and proliferating cell nuclear antigen (PCNA) expression were investigated in normal hearts, in hypertrophic from hemodynamic overload hearts and in hypertrophic cardiomyopathy (HCM). The aim of this study was mainly to determine whether the hyperdiploid myocardial cells in all cases are in dynamic or static phase.
The percentage of PCNA positive cells only in the HCM group was significantly higher (mean value=25.4%) than the percentage of hyperdiploid cells (mean value=9.3%). Therefore, the DNA replication occurs through a different process from that of normal cell cycle which lead to an increase in ploidy and eventually mitosis. These data should be interpreted not only as the result of a periodic amitotic DNA renewal and not even as the result of an increased apoptosis, but especially as a repair process of the DNA molecules affected by a various types of damages in HCMs.
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References
Anversa P, Palackal T, Sonnenblick EH et al. (1990) Hypertensive cardiomyopathy. J Clin Invest 85: 994–997
Clubb FJ, Bishop SP (1984) Formation of binucleated myocardial cells in the neonatal rat. Lab Invest 50: 571–577
Galand P, Degraef C (1989) Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labeling for making S phase cells in paraffin sections from animal and human tissue. Cell Tissue Kinet 22: 383–392
Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM (1986) Expression of Proliferating Cell Nuclear Antigen (PCNA/Cyclin) during the cell cycle. Exp Cell Res 166: 209–219
Marino T, Haldar S, Williamson C, Beaverson K et al. (1991) Proliferating cell nuclear antigen in developing and adult rat cardiac muscle cells. Circulation Research 69: 1353–1360
Mathews MB, Bernstein RM, Franza BR, Garreis JL (1984) Identify of the proliferating cell nuclear antigen and cyclin. Nature 309: 374–376
Matturri L, Lavezzi AM (1992) Proliferation in human tumors: evaluation of methods and applications as prognostic variables. Anal Cell Pathol 4: 199
Matturri L, Lavezzi AM, Riberti C, Vercesi F, Azzolini A (1992) Long-term histopathologic evaluation of human expanded skin. Plastic Reconstr Surg 90: 636–642
Matturri L, Biondo B, Lavezzi AM, Rossi L (1994) Evaluation of DNA content by static citometry in hypertrophic cardiomyopathy. Abstract Int Workshop on Cardiac Growth and Regeneration, 85
Matturri L, Lavezzi AM, Grignani F, Roviaro GC (1994) The prognostic value of cell proliferation in non-small cell lung cancer assessed with tritiated thymidine and anti-PCNA antibodies. Eur J Cancer 30: 1397–1398
Matturri L, Biondo B, Lavezzi AM (1995) Cell kinetics, DNA content and chromosome aneuploidy as prognostic indicators in human tumours. A critical apparaisal. Cancer Detect Prev 19: 80
Matturri L, Biondo B, Grosso F, Lavezzi AM, Rossi L (1995) Morphometric and densitometric approach in hypertrophic cardiomyopathy. Eur J Histochem 39: 237–244
Pelc SR (1964) Labelling of DNA and cell division in so called non-dividing tissues. J Cell Biol 22: 21–28
Peterson RO, Baserga R (1965) Nucleic acid and protein synthesis in cardiac muscle of growing and adult mice. Exp Cell Res 40: 340–352
Pfitzer P (1971) Nuclear DNA content of human myocardial cells. Curr Top Pathol 54: 125–168
Rumayantsev PP (1977) Interrelations of the proliferation and differentiation process during cardiac myogenesis and regeneration. Int Rev Cytol 51: 187–273
Sandritter W, Scomazzoni G (1964) Deoxyribonucleic acid content (Feulgen photometry) and dry weight (interference microscopy) of normal and hypertrophied heart muscle fibers. Nature 202: 100–101
Anversa P, Fitzpatrick D, Argani S, Capasso JM (1991) Myocyte mitotic division in the aging mammalian rat heart. Circ Res 69: 1159–1164
Kaistura J, Zhang X, Reiss K, Szoke E, Li P, Lagrasta C, Cheng W, Darzynkiewicz Z, Olivetti G, Anversa P (1994) Myocyte cellular hyperplasia and myocyte hypertrophy contribute to chronic ventricular remodeling in coronary artery narrowing-induced cardiomyopathy in rats. Circ Res 74: 383–400
Quaini F, Cigola E, Lagrasta C, Saccani G, Quaini E, Anversa P (1994) Endstage cardiac failure in humans is coupled with the induction of proliferating cell nuclear antigen and nuclear mitotic division in ventricular myocytes. Circ Res 75: 1050–1063
Silvestrini R, Matturri L (1990) Cell kinetics and morphological criteria of malignant tumors. In: General Surgery-Current Status and Future Trends. Raven Press, pp 461–466
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Matturri, L., Biondo, B., Colombo, B. et al. Significance of the DNA synthesis in hypertrophic cardiomyopathies. Basic Res Cardiol 92, 85–89 (1997). https://doi.org/10.1007/BF00805568
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DOI: https://doi.org/10.1007/BF00805568