Action of allogeneic and syngeneic splenic extracts on primary cell cultures from inbred mice

Abstract

The effect of allogeneic and syngeneic extracts from the spleens of male and female inbred mice on primary cultures of fibroblasts obtained from the subcutaneous connective tissues of fetuses of CBA and C57BL/6J mice was studied. The cytotoxic and growth-inhibiting action on the cultures was successively enhanced by the use of extracts from syngeneic male and allogeneic female and male tissues. Consequently, an increase in the degree of antigenic difference between the target cells and extracts led to enhancement of the phenomenon of allogeneic inhibition. It was shown for the first time that in a syngeneic system extracts from male tissues (containing the weak H-Y antigen) have a cytotoxic action on cells from female inbred mice, i.e., that they induce a reaction of the allogeneic inhibition type.