Abstract
Structural and functional changes in lysosomes of cells in the regional lymph nodes and liver were investigated in the course of toxic hepatitis caused by single or repeated administration of CCl4 to dogs. Total activity of the lymph node lysosomal enzymes studied (β-galactosidase, acid RNase, cathepsin D) exceeded the corresponding activity in the liver of intact dogs, and this reflects the barrier or protective function of the organ. With the development of acute toxic hepatitis a sharp increase was found in acid RNase and cathepsin D activity. At the times of testing (8 and 30 days) the parameters studied had not returned to normal. Meanwhile the mass of the regional lymph nodes and the relative number of macrophages and neutrophils in their sinuses were increased. The increase in lysosomal enzyme activity in the regional lymph nodes following liver damage is connected with the increased functional load on the lymph nodes, which are involved in the hydrolysis of biopolymers brought to the regional lymph nodes with the lymph flow.
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Department of Normal Anatomy and Central Research Laboratory, Novosibirsk Medical Institute. Translated from Byulleten' Éxperimental'noi Biologii i Meditsiny, Vol. 86, No. 10, pp. 428–431, October, 1978.
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Borodin, Y.I., Korolenko, T.A., Malygin, A.E. et al. Structural and functional changes in lysosomes of regional lymph nodes and liver of dogs with toxic hepatitis. Bull Exp Biol Med 86, 1317–1320 (1978). https://doi.org/10.1007/BF00800446
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DOI: https://doi.org/10.1007/BF00800446