Abstract
A method of investigation of synthesis of two different substances (RNA and protein) in the same cell is described. Two specific precursors, uridine and proline, each labeled with tritium, were injected into animals. Differentiation of RNA and protein labeling was based on the fact that the biosynthesis of these macromolecules is located in different parts of the cell. Comparison of the results with those of two control experiments in which only one precursor was used showed that the suggested method is suitable for a simultaneous study of RNA and protein synthesis in wound fibroblasts.
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Pal'tsyn, A.A., Kolokol'chikova, E.G. A double labeling method in electron-microscopic autoradiography. Bull Exp Biol Med 86, 1550–1553 (1978). https://doi.org/10.1007/BF00800311
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DOI: https://doi.org/10.1007/BF00800311