Abstract
The proliferative activity of precursors of bone marrow stromal cells forming colonies (clones of fibroblasts) in monolayer cultures was studied by the thymidine-suicide method in vitro. Clonogenic cells of native bone marrow were shown not to be inhibited by thymidine-H3 with high specific activity, i.e., they virtually do not proliferate in vivo. Having started to proliferate 24 h after explantation, they then give rise to colonies of fibroblasts. In 6–14-day bone marrow cultures 39±4% of clonogenic cells die under the influence of thymidine-H3. Hence it follows that, unlike precursor cells in native bone marrow, clonogenic cells in primary cultures proliferate actively.
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Epikhina, S.Y., Latsinik, N.V. Proliferative activity of clonogenic bone marrow stromal precursor cells. Bull Exp Biol Med 81, 67–69 (1976). https://doi.org/10.1007/BF00800170
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DOI: https://doi.org/10.1007/BF00800170