Abstract
The morphological characteristics of primary monolayer cultures obtained from the bovine fetal pancreas are described. Dispersion of the pancreatic tissue was achieved by combined treatment with solutions of trypsin and collalytin, a preparation with collagenase activity. The cultures thus obtained included many epithelial cells corresponding in their morphological and functional characteristics to β cells of the islets of Langerhans: Aldehyde-fuchsinophilic granules were found in their cytoplasm; degranulation of these cells took place if the glucose concentration in the nutrient medium was increased.
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Literature Cited
I. S. Turchin, “The study of the structure and function of the cells of endocrine glands by the tissue culture method,” Author's Abstract of Doctoral Dissertation, Kiev (1975).
W. F. Ballinger and P. E. Lacy, Surgery,72, 175 (1972).
W. L. Chick, V. Lauris, J. H. Flewelling, et al., Endocrinology,92, 212 (1973).
W. L. Chick, A. A. Like, and V. Lauris, Science,187, 847 (1975).
I. Hilwig, S. Schuster, W. Heptner, et al., Z. Zellforsch.,90, 333 (1968).
P. E. Lacy and M. Kostianowsky, Diabetes,16, 35 (1967).
A. E. Lambert, B. Blondel, J. Kanazawa, et al., Endocrinology,90, 239 (1972).
S. Moskalewski, Gen. Comp. Endocrinol.,5, 342 (1965).
C. Weber, and R. Weil, R. McIntosh, et al., Surgery,77, 208 (1975).
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Babikova, R.A., Blyumkin, V.N., Shal'nev, B.I. et al. Cell cultures from the bovine fetal pancreas obtained by the use of collalytin. Bull Exp Biol Med 83, 403–405 (1977). https://doi.org/10.1007/BF00799377
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DOI: https://doi.org/10.1007/BF00799377