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An improved method for the determination of glycosyltransferases usingpara-nitrophenyl-glycosides as substrates

Eine verbesserte Methode zur Bestimmung von Glykosyltransferase-Aktivitäten unter Verwendung von Nitrophenyl-Glykosiden als Substrate

  • Organische Chemie Und Biochemie
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Abstract

Due to their easy purification nitrophenyl-glycosides have become of increasing interest as acceptor substrates for the assay of glycosyltransferase activity. In this communication reversed-phase chromatography is shown to provide a convenient means for the isolation of the radioactively labelled nitrophenylglycoside(s) formed in this reaction, superior to the methods hitherto used with regard to speed and specifity.

Zusammenfassung

Nitrophenylglykoside werden immer häufiger als Akzeptor-Substrate bei der Bestimmung von Glykosyltransferase-Aktivitäten verwendet, da sie leicht aus dem Reaktionsgemisch zu isolieren sind. In der vorliegenden Mitteilung wird gezeigt, daß die „reversed-phase“-Chromatographie eine bequeme Möglichkeit darstellt, die bei der Reaktion entstehenden radioaktiv markierten Nitrophenylglykoside zu reinigen. Sie ist den bisher verwendeten Trennmethoden an Geschwindigkeit und Spezifität überlegen.

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Authors and Affiliations

  1. Institut für Biochemie, Universität Wien, A-1090, Vienna, Austria

    Christian G. Haselberger, Diethelm J. Gauster & Helmut Schenkel-Brunner

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  1. Christian G. Haselberger
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  2. Diethelm J. Gauster
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  3. Helmut Schenkel-Brunner
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Haselberger, C.G., Gauster, D.J. & Schenkel-Brunner, H. An improved method for the determination of glycosyltransferases usingpara-nitrophenyl-glycosides as substrates. Monatsh Chem 114, 625–630 (1983). https://doi.org/10.1007/BF00798618

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  • DOI: https://doi.org/10.1007/BF00798618

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