Abstract
A highly sensitive electroimmunodiffusion method of detecting antigens on cellulose acetate films is suggested. There are three stages: concentration of the antigen in a discontinuous buffer system on cellulose acetate films; detection of the antigen on the same films by immunodiffusion using a standard test systems; staining the washed films with a protein stain to detect precipitation bands if the reaction takes place in the visible zone, or further treatment by “growing” precipitates with antiglobulin antibodies or by autoradiography. The method can detect nanogram amounts of α-fetoprotein and can be used to discover antigens of different molecular weight and electrophoretic mobility.
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Literature Cited
G. I. Abelev, Byull. Éksp. Biol. Med., No. 11, 120 (1973).
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D. A. Él'gort and G. I. Abelev, Byull. Éksp. Biol. Med., No. 2, 118 (1971).
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Abelev, G.I., Sokolenko, A.A. A highly sensitive electroimmunodiffusion method of antigen detection on cellulose acetate films. Bull Exp Biol Med 84, 1065–1068 (1977). https://doi.org/10.1007/BF00798548
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DOI: https://doi.org/10.1007/BF00798548