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32P-labeling of bovine tryptophanyl-tRNA synthetase with [32P]pyrophosphate

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Abstract

The covalent derivative of the tryptophanyl-tRNA synthetase obtained under the action of32PPi contains one mole of the covalently bound pyrophosphate (or 2 moles of orthophosphate) per mole of dimeric enzyme. Dephosphorylation with alkaline phosphatase causes practically no changes of enzymatic activity although the enzyme looses its ability to bind PPi.

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Abbreviations

Enzymes:

tryptophanyl-tRNA synthetase (EC 6.1.1.2), alkaline phosphatase (EC 3.1.3.1), inorganic pyrophosphatase (EC 3.6.1.1)

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Authors and Affiliations

  1. Institute of Molecular Biology, USSR Academy of Sciences, V-334, Moscow, USSR

    G. K. Kovaleva, S. Ch. Degtyarev & L. L. Kisselev

Authors
  1. G. K. Kovaleva
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  2. S. Ch. Degtyarev
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  3. L. L. Kisselev
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Kovaleva, G.K., Degtyarev, S.C. & Kisselev, L.L. 32P-labeling of bovine tryptophanyl-tRNA synthetase with [32P]pyrophosphate. Mol Biol Rep 8, 17–20 (1981). https://doi.org/10.1007/BF00798379

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  • DOI: https://doi.org/10.1007/BF00798379

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