Abstract
DNA-, RNA- and protein synthesis have been studied inMycobacterium smegmatis cells infected with phage 13. The macromolecular synthesis continued until the end of latent period. Early RNA and protein synthesis were necessary prior to the commencement of DNA replication. The infecting phage DNA sedimented as larger than unit length of genome, after initiation of DNA synthesis. Although the host DNA was not degraded, 90 percent of the RNA synthesized after phage infection hybridized to phage DNA.
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BotsteinD., 1968. J. Mol. Biol. 34: 621–641.
BotsteinD. & LevineM., 1968. Cold Spring Harbor Symp. Quant. Biol. 33: 659–667.
GadagkarR. & GopinathanK. P., 1980. J. Biosci. 2: 337–348.
GillespieD. & SpiegelmanS., 1965. J. Mol. Biol. 12: 829–842.
GopinathanK. P., SarojaD., GadagkarR. R. & RamakrishnanT., 1978. In: E.Freerksen, I.Tarnok & J. H.Thumim (eds.). Genetics of Actinomycetales, Fisher, Stuttgart, New York. pp. 237–242.
LewinB., 1977. Gene expression −3. In: Plasmids and phages. Wiley, New York.
O'FarrellP. Z. & GoldL. M., 1973. J. Biol. Chem. 248: 5502–5511.
NagarajaV. & GopinathanK. P., 1980. Arch. Microbiol. 124: 249–254.
SiegelP. J. & SchaechterM., 1973. Annu. Rev. Microbiol. 27: 261–282.
SummersW. C., 1970. Anal. Biochem. 33: 459–463.
SunderrajC. V. & RamakrishnanT., 1970. Nature 228: 280–281.
WilhelmJ. & HaselkornR., 1971. In: Methods in Enzymology (K.Moldave and L.Grossman, eds.) Acad. Press. New York, 20, part C, pp. 531–536.
WitmerH. J., 1971. J. Biol. Chem. 246: 5220–5227.
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Nagaraja, V., Gopinathan, K.P. Macromolecular synthesis in mycobacteriophage I3 infected cells. Mol Biol Rep 8, 11–15 (1981). https://doi.org/10.1007/BF00798378
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DOI: https://doi.org/10.1007/BF00798378