Summary
Mitochondria prepared from rainbow trout liver consistently display high respiratory control and ADP/O ratios. These appear to possess a complete Krebs cycle, since pyruvate, palmitoyll-carnitine, citrate, and various Krebs cycle intermediates can all be oxidized. Rapid oxidation of pyruvate and palmitoyll-carnitine requires the pressence of malate. Oxidation of palmitoyll-carnitine appears to inhibit pyruvate oxidation. Malate stimulates α-ketoglutarate oxidation while aspartate inhibits glutamate oxidation, indicating the presence of malate-α-ketoglutarate and glutamate-aspartate carriers. These properties are compared with those of liver mitochondria from other species of fish.
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References
Bilinski E, Jonas REE (1970) Effects of coenzyme A and carnitine on fatty acid oxidation by rainbow trout mitochondria. J Fish Res Board Can 27:857–864
Bremer J (1966) Comparison of acylcarnitines and pyruvate as substrates for ratliver mitochondria. Biochem Biophys Acta 116:1–11
Brown WD, Tappel AL (1959) Fatty acid oxidation by carp liver mitochondria. Arch Biochem Biophys 85:149–158
Cederbaum AI, Lieber CS, Beattie DS, Rubin E (1973) Characterization of shuttle mechanisms for the transport of reducing equivalents into mitochondria. Arch Biochem Biophys 158:763–781
Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol 17:65–134
Chappell JB (1964) The oxidation of citrate, isocitrate and cisaconitate by isolated mitochondria. Biochem J 90:225–237
Chappell JB (1968) Systems used for the transport of substrates into mitochondria. Br Med Bull 24:150–157
Estabrook RW (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. In: Colowick SP, Kaplan NO (eds) Methods of enzymology, vol 10. Academic Press, New York, pp 41–47
French CJ, Mommsen TP, Hochachka PW (1981) Amino acid utilization in isolated hepatocytes from rainbow trout. Eur J Biochem 113:311–317
Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766
Gumbmann M, Tappel AL (1962) The tricarboxylic acid cycle in fish. Arch Biochem Biophys 98:262–270
Hayashi S, Ooshiro Z (1977) Gluconeogenesis in perfused eel liver-Effect of starvation, amino-oxyacetate, D-malate, and hormones. Mem Fac Fish Kagoshima Univ 26:89–95
Hayashi S, Ooshiro Z (1979) Gluconeogenesis in isolated liver cells of the eel,Anguilla japonica. J Comp Physiol 132:343–350
Hazel JR, Sellner PA (1979) Fatty acid and sterol synthesis by hepatocytes of thermally acclimated rainbow trout (Salmo gairdneri). J Exp Zool 209:105–114
Hochachka PW (1968) Action of temperature on branch points in glucose and acetate metabolism. Comp Biochem Physiol 25:107–118
Kun E (1963) Malate dehydrogenases In: Boyer PD, Lardy H, Myrback K (eds). The enzymes, vol 7. Academic Press, New York, pp 149–160
LaNoue KF, Duszynski J, Watts JA, McKee E (1979) Kinetic properties of aspartate transport in rat heart mitochondrial inner membranes. Arch Biochem Biophys 195:578–590
Moon TW, Quellet G (1979) The oxidation of tricarboxylic acid cycle intermediates, with particular reference to isocitrate, by intact mitochondria isolated from the liver of the American eel,Anguilla rostrata LeSuer. Arch Biochem Biophys 195:438–452
Newsholme EA, Start C (1973) Regulation in metabolism. Wiley, London
Phillips JW, Hird FJR (1977a) Gluconeogenesis in vertebrate livers. Comp Biochem Physiol 57B:127–131
Phillips JW, Hird FJR (1977b) Ketogenesis in vertebrate livers. Comp Biochem Physiol 57B:133–138
Renaud JM, Moon TW (1980) Characterization of gluconeogenesis in hepatocytes isolated from the American eel,Anguilla rostrata LeSeur. J Comp Physiol 135:115–125
Slater EC, Welle HF (1969) Applications of oligomycin and related inhibitors in bioenergetics. In: Bücher T, Sies H (eds) Inhibitors. Tools in cell research. Springer, Berlin Heidelberg New York, pp 258–278
Smith CL (1973) temperature dependence of oxidative phosphorylation and of the activity of various enzyme systems in liver mitochondria from cold and warm-blooded animals. Comp Biochem Physiol 46B:445–461
Suarez RK, Hochachka PW (1981) The pyruvate branch point in fish liver mitochondria: Effects of acylcarnitine oxidation on pyruvate dehydrogenase and pyruvate carboxylase activities. J Comp Physiol 143:275–279
Walton MJ, Cowey CB (1979) Gluconeogenesis by isolated hepatocytes from rainbow troutSalmo gairdneri. Comp Biochem Physiol 62B:75–79
Williamson JR, Safer B, LaNoue KF, Smith CM, Walajtys E (1973) Mitochondrial-cytosolic interactions in cardiac tissue: Role of the malate-aspartate cycle in the removal of glycolytic NADH from the cytosol. Soc Exp Biol Symp 27:241–281
Wodtke E (1974) Properties of isolated mitochondria of the eel and their dependency on acclimation temperature with special regard to oxidative phosphorylation. J Comp Physiol 91:277–307