Skip to main content
SpringerLink
Log in

Cytochemical investigation of the alkaline phosphatase activity of the leukocytes after injection of pyrogenal

  • Pathological Physiology and General Pathology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Summary

A cytochemical method of azocombination was used to study the activity of alkaline phosphatase of human leukocytes after injection of pyrogenal (bacterial lipopolysaccharide). Intravenous injection of pyrogenal at first causes a decline and then an increase of the phosphatase activity of neutrophils attaining a maximum on the 3rd–4th day, after which the activity returns to normal. No direct relationship has been discovered between the phosphatase activity of neutrophils and the number of these cells in the peripheral blood. It is presumed that the growth of the phosphatase activity of neutrophils of the peripheral blood is connected with supply of cells rich in alkaline phosphatase from the bone marrow.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. P. Z. Budnitskaya, In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 8.

  2. P. Z. Budnitskaya, The Isolation, Chemical Characteristics, and some Biological Properties of a Preparation of Pyrogenal, Candidate dissertation, Moscow (1963).

  3. K. I. Gladkova and I. N. Ternovskii, In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 208.

  4. O. Sh. Dzhesenbaev, In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 24.

  5. E. N. Iordanskaya and T. N. Nesmeyanova, In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 34.

  6. T. S. Istamanova and V. A. Almazov, Leukopenias and Agranulocytoses [in Russian], Leningrad (1961).

  7. S. M. Mebel' (Mushkatblat), In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 50.

  8. É. V. Montsevichyute-Eringene, Pat. Fiziol., No. 4 (1964), p. 71.

    Google Scholar 

  9. Kh. Kh. Planel'es, In the book: Experimental Investigations and Clinical Application of Pyrogenal [in Russian], Moscow (1961), p. 5.

  10. T. R. Petrova and M. G. Shubich, Ter. Arkh., No. 9 (1964), p. 29.

    Google Scholar 

  11. V. E. Predtechenskii, Textbook of Clinical Laboratory Investigations [in Russian], Moscow (1960), p. 27.

  12. M. G. Shubich, In the book: Proceedings of the 7th Jubille Practical Scientific Conference of Health Resort Physicians to Commemorate the 10th Anniversary of the “Gorachii Klyuch” Health Resort [in Russian], Goryachii Klyuch (1964), p. 117.

  13. M. G. Shubich and A. A. Ashman, Zh. Nevropat. Psikhiat., No. 1 (1965), p. 29.

    Google Scholar 

  14. M. G. Shubich, Lab. Delo, No. 1 (1965), p. 10.

    Google Scholar 

  15. D. N. Yanovskii, The Blood Picture and Its Clinical Importance [in Russian], Kiev (1957).

  16. G. Cartright, G. Athens, and M. Wintrobe, Blood, Vol. 24 (1964), p. 780.

    Google Scholar 

  17. T. Fliedner, E. Cronkite, and T. Robertson, Ibid., p. 402.

    Google Scholar 

  18. G. Frick and H. Konrad, Folia Haemat., Vol. 81, No. 374, Leipzig (1964).

  19. F. Jacob and J. Monod, Proceedings of the 5th International Biochemical Congress. Symposium 1. Biological Structures and Functions at the Molecular Level [in Russian], Moscow (1962), p. 157.

  20. L. Kaplow, Blood, Vol. 10 (1955), p. 1023.

    Google Scholar 

  21. K. Lambers, Z. Klin Med., Vol. 155, No. 176 (1958).

  22. H. Merker and L. Heilmeyer, Dtsch. Med. Wschr., Vol. 85, No. 253 (1960).

  23. H. Merker, Folia Haemat., Vol. 6, No. 153, Frankfurt (1961).

  24. W. Plenert, Folia Haemat, Vol. 80, No. 52, Leipzig (1963).

  25. D. Remy, Anat. Ges., Vol. 57, No. 18, Jena (1963).

  26. Takuda Taneji, Tsujimura Hishashi, Ikeda Jositern et al., J. Electron Microsc. Vol. 10 (1961), p. 161.

    Google Scholar 

  27. S. Trubowitz, D. Feldman, C. Benannite, et al., Am. J. Clin. Path., Vol. 39 (1959). p. 481.

    Google Scholar 

  28. W. Valentine, J. Follette, D.Solomon, et al., In the book: J. W. Rebuck et al., (Ed.), The Leukemias — Etiology, Pathophysiology, and Treatment, New York (1957), p. 457.

  29. F. Wendt and C. Budde, Folia Haemat., Vol. 6, No. 149, Frandfurt (1961).

Download references

Author information

Authors and Affiliations

  1. Department of Histology and Faculty of Therapy, Kuban Medical Institute, Krasnodar

    M. G. Shubich, K. I. Gladkova & A. D. Vakulenko

  2. Department of Psychiatry, Daghestan Medical Institute, Krasnodar

    M. G. Shubich, K. I. Gladkova & A. D. Vakulenko

Authors
  1. M. G. Shubich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. I. Gladkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. D. Vakulenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shubich, M.G., Gladkova, K.I. & Vakulenko, A.D. Cytochemical investigation of the alkaline phosphatase activity of the leukocytes after injection of pyrogenal. Bull Exp Biol Med 62, 1124–1126 (1966). https://doi.org/10.1007/BF00795455

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00795455

Keywords

Search

Navigation