Summary
A fraction of urine isolated by means of ion-exchange chromatography caused enhanced uptake of glucose by rat diaphragm and epididymal fat pad, and intensified respiration of the latter. Concentrated solutions of the fraction induced hypoglycemic convulsions in mice. The yield of activity in the urine rose following administration of glucose to healthy individuals. The activity was not abolished by treatment with acids, but was abolished by alkali treatment, and by heating at 100°, as well as by thiols. On these criteria, it is concluded that the urinary fraction examined contained insulin.
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Liberman, L.L. Evidence of the presence of insulin in urine. Bull Exp Biol Med 55, 639–641 (1964). https://doi.org/10.1007/BF00786804
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DOI: https://doi.org/10.1007/BF00786804