Summary
A modification of the Nauta method of impregnation of degenerated axons is described. With this method, the quality of axon impregnation depends upon a number of factors which can only be determined experimentally during staining. On this account impregnation may be uhsuccessful. The Nauta method is not applicable to a study of serial brain sections because of the comparatively small number of sections which can be treated. The essential advantages of the described modification are the consistency of the results, the greater number of sections which can be handled, and the considerable economy of silver.
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W. Nauta, Selective silver impregnation of degenerating axons in the central nervous system, Stain Technology, 1952, Vol. 27, No. 3, p. 175.
W. Nauta. Selective silver impregnation of degenerating axons in the central nervous system, Stain Technology, 1954, Vol. 29, No. 2, p. 91.
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Zambrzhitskii, I.A. Modification of Nauta's method. Bull Exp Biol Med 55, 468–470 (1964). https://doi.org/10.1007/BF00785682
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DOI: https://doi.org/10.1007/BF00785682