Summary
As a result of observations conducted for many years, the authors have developed a method of obtaining avirulent and high immunogenic strains ofShigella flexneri. The principle of this method consists of cultivating the microorganisms in cold-blooded animals (pond frogs) resistant to this infection, as well as on animal products (60% bile-peptone broth).
An usual stability of the acquired properties, which is not characteristic for this species of bacteria, was established. 3 experimental strains appeared to be avirulent, and highly immunogenic and retained all the biological properties. The possibility of studying dysentery immunization problems on young cats was demonstrated.
LivingShigella flexneri vaccine possesses immunological efficacy in experiments on this species of animals; it may be stored in a refrigerator up to 9 months without losing its properties.
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Literature Cited
V. V. Budylina, A. Yu. Illyutovich, Z. S. Petrova et al., Byull. Eksptl. Biol. i Med., 43, 2, 70 (1957).
Yu. A. Belaya, Zhur. Mikrobiol., Épidemiol. i Immunobiol., No. 4, 77 (1958).
A. Yu. Illyutovich, Z. S. Petrova, E. E. Golubeva et al., Proceedings of an Inter-Institute Conference on Strains, Standards and Diagnostic Preparations, p. 12, Moscow, 1957 [In Russian].
A. Yu. Illyutovich, Z. S. Petrova, Collected Scientific Transactions of the Stavropol Research Institute of Vaccines and Sera, 4, p. 125, Stavropol, 1957 [In Russian].
A. A. Kashaeva, Proceedings of an Inter-Regional Scientific and Practical Conference on the Problem of Enteric Infections, p. 20, Rostov-on-Don, 1955 [In Russian].
I. L. Kovalevskaya, Zhur. Mikrobiol., Épidemiol. i Immunobiol., No. 7, 10 (1955).
E. I. Korobkova, Living Plague Vaccine, Moscow, 1956 [In Russian].
S. V. Lesnyak, Zhur. Mikrobiol. Épidemiol. i Immunobiol., No. 3, 53 (1952).
D. G. Manolov, Z. S. Petrova, Collected Scientific Transactions of the Stavropol Research Institute of Vaccines and Sera, 3, p. 33, Stavropol, 1954 [In Russian].
I. N. Morgunov, S. L. Yagud, and N. A. Maksimovich, Theses of Scientific Works of the Institute of Epidemiology and Microbiology in 1955, p. 7 Kiev, 1956 [In Russian].
E. I. Novikova, G. A. Lalazarov, Vestnik Mikrobiol. Épidemiol. i Parazitol., 19, 2, 271 (1940).
M. M. Priselkov, E. N. Malikova, S. V. Lesnyak et al., Byull. po Obmeny Opytom Raboty Institutov Épidemiol. i Mikrobiol., No. 2/40, 13 (1952).
N. N. Sirotinin, I. V. Ovsievskaya, E. A. Brodskaya et al., Zhur. Mikrobiol., Épidemiol. i Immnobiol., No. 3, 14 (1958).
E. A. Sergeevich, Zhur. Mikrobiol., Épidemiol. i Immunobiol., No. 4, 71 (1954).
V. M. Zhdanov, Living Vaccines, vol. 2, p. 5, Moscow, 1956 [In Russian].
F. S. Shulyak, The Action of Bile on Certain Species of Pathogenic Microorganisms. Author's abstract of dissertation, Moscow, 1951 [In Russian].
S. R. Khomik, Investigations in Hygiene and Sanitation: Enteric Infections, Leptospirosis, p. 142. Rostov-on-Don, 1956 [In Russian].
B. Ya. El'bert, Zhur. Mikrobiol., Épidemiol. i Immunobiol., No. 11, 40 (1957).
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From the Stavropol Institute of Vaccines and Sera (Director-Candidate Medi. Sci. V. M. Kruglikov)
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Illyutovich, A.Y., Petrova, Z.S., Chetvernika, R.S. et al. The experimental production of vaccine strains of shigella flexneri and the study of the immunological effectiveness of a living dysentery vaccine. Bull Exp Biol Med 48, 1248–1254 (1959). https://doi.org/10.1007/BF00785183
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DOI: https://doi.org/10.1007/BF00785183