Intensity of the synthesis of nonspecific proteins of the organism under the influence of the lipopolysaccharide complex from typhoid bacillus

Summary

Lipopolysaccharide complex of typhoid bacilli was injected intravenously to chinchilla rabbits. The dose of the antigen was titrated according to the pyrogenic effect and the capacity for inducing the antibody production. Each rabbit received a stimulating antibody-producing dose of 15 γ and an immunizing dose of 3.5–5 γ of the antigen per kg of the animal's body weight.

Methionine S³⁵ was used as a radioaminoacid. It was administered to experimental animals 4 h after the antigen had been introduced. Control rabbits received only methionine S³⁵.

It was experimentally shown that administration of lipopolysaccharide complex (in various doses) stimulated, during the first hours, the synthesis intensity in proteins of blood serum, in its albumin and globulin fractions, and in proteins of the liver, spleen, adrenal glands, as well as in proteins of the regional lymph nodes (retroperitoneal). No synthesis activation was observed in distal lymph nodes.

Simultaneously with the activation of the intensity of the body protein synthesis there occurred a marked plasmocytic reaction in the regional lymph nodes and the spleen.