Summary
A method of using microcapillaries filled with a small amount of nutrient medium is suggested for trapping and planting single cells in test-tubes. Trapping is done from a drop of cellular suspension on a slide, which is fixed under a microscope. Since the time of cloning is minimized cloning, cellular injury decreases, and the planting efficiency rises. The yield of colonies for A-1 strain was 63%, HeLa strain −50%, No. 630 strain −37% and Cave strain −10%. The number of planted single cells is unlimited. The use of test tubes simplified obtaining of clonal cell lines.
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Ugryumov, E.P. A method of obtaining clone lines of cells. Bull Exp Biol Med 57, 369–370 (1964). https://doi.org/10.1007/BF00781931
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DOI: https://doi.org/10.1007/BF00781931