Skip to main content
SpringerLink
Log in

The accessibility of phage MS2 RNA to structure specific nucleases in various conditions

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The accessibility of ds-and ss-segments of phage MS2 RNA to ds-and ss-specific nucleases (RNase III, nuclease SV and nuclease S1) was studied. The results show that the RNA has hydrolysis sites for all the nucleases used. These sites are unvariable in a wide range of the conditions (ionic strength, pH, bivalent cations and temperature) and are not changed also after denaturation-renaturation of the RNA. This testifies that the distribution and interactions of ds-and ss-segments in the whole molecule are very specific and stable.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vogt, V. M., 1973. Eur. J. Biochem. 33: 192–200.

    Google Scholar 

  2. Vassilenko, S. K. & Rite, V. K., 1975. Biochimia (Russ.) 40: 578–582.

    Google Scholar 

  3. Robertson, H. D., Webster, R. E. & Zinder, N. D., 1968. J. Biol. Chem. 243: 82–91.

    Google Scholar 

  4. Krol, A., Gallinaro, H., Lazar, E., Jakob, M. & Branlant, C., 1981. Nucl. Acids Res. 9: 767–787.

    Google Scholar 

  5. Wrede, P., Wurst, R., Vournakis, J. & Rich, A., 1979. J. Biol. Chem. 254: 9608–9616.

    Google Scholar 

  6. Peackock, A. C. & Dingman, C. N., 1967. Biochem. 6: 1818–1830.

    Google Scholar 

  7. Senchenko, V. N., Jakovlev, G. I., Kolbanovskaja, E. Yu., Smirnoff, I. V. & Karpeiski, M. Ya., 1981. Bioorgan. Chimia (Russ.) 7: 1346–1350.

    Google Scholar 

  8. Grechko, V. V., Tymokhina, G. I. & Aleshkina, L. A., 1982. Molekularnaja Biologia (Russ.) 16: 1097–1105.

    Google Scholar 

  9. Kramer, R. A. & Rosenberg, M., 1976. Nucl. Acids Res. 3: 2411–2426.

    Google Scholar 

  10. Westphal, H. & Crouch, R. J., 1975. Proc. Natl. Acad. Sci. USA 72: 3077–3082.

    Google Scholar 

  11. Goton, S., Nikolaev, N., Battaner, E., Birge, C. H. & Schlessinger, D., 1974. Biochem. Biophys. Res. Communs. 59: 972–978.

    Google Scholar 

  12. Mitra, S., Enger, M. D. & Karlsberg, P., 1963. Proc. Natl. Acad. Sci. USA. 50: 68–75.

    Google Scholar 

  13. Strauss, J. K. & Sinsheimer, R. L., 1963. J. Mol. Biol. 7: 43–54.

    Google Scholar 

  14. Min You, W., Haegeman, G., Ysebaert, M. & Fiers, W., 1972. Nature 237: 82–88.

    Google Scholar 

  15. Fiers, W., Contreras, R., Duerinck, F., Haegeman, G., Merregaert, J., Min You, W., Raeymakers, A., Volckaert, G., Ysebaert, M., Vandekerckhove, J., Nolf, F. & Vanmontagu, M., 1975. Nature 256: 273–278.

    Google Scholar 

  16. Fiers, W., Contreras, R., Duerinck, F., Haegeman, G., Iserentant, D., Merregaert, J., Min You, W., Molemans, F., Raeymakers, A., Van den Berghe, A., Volckaert, G. & Ysebaert, M., 1976. Nature 260: 500–507.

    Google Scholar 

  17. Nomoto, A., Lee, Y. F., Babich, A., Jakobson, A., Dunn, J. J. & Wimmer, E. R., 1979. J. Mol. Biol. 128: 165–177.

    Google Scholar 

  18. Wertz, G. W. & Davis, N., 1981. Nucl. Acids Res. 9: 6487–6504.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the USSR, Vavilova str. 32, 117984, Moscow, USSR

    V. V. Grechko, G. I. Tymokhina & L. A. Aleshkina

  2. All-Union Scientific Research Institute of Molecular Biology, Koltzovo, 630090, Novosibirsk, USSR

    J. I. Kalashnikova, S. P. Majev & S. K. Vassilenko

Authors
  1. V. V. Grechko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. I. Tymokhina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. A. Aleshkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. I. Kalashnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. P. Majev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. K. Vassilenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grechko, V.V., Tymokhina, G.I., Aleshkina, L.A. et al. The accessibility of phage MS2 RNA to structure specific nucleases in various conditions. Mol Biol Rep 10, 137–142 (1985). https://doi.org/10.1007/BF00778518

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00778518

Keywords

Search

Navigation