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Internation of zinc ions with DNA-dependent RNA polymerases A, B and C isolated from calf thymus

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Abstract

Purified DNA-dependent RNA polymerases A, B and C isolated from calf thymus contain a significant amount of zinc. Atomic absorption spectroscopy revealed the presence of 6.7, 5.35 and 2.6–4.1 g-atoms of zinc per mole of polymerase A, B and C, respectively. These enzymes are inhibited by treatment with 1,10-phenanthroline at concentrations varying from 10-5 to 10-4 M. However, the addition of zinc ions does not restore fully the activity of 1,10-phenanthroline treated enzymes. Exogenous zinc ions reducein vitro an overall RNA synthesis catalysed by RNA polymerases from calf thymus. In addition to the sites which bind zinc in a specific and stoichiometric way these enzymes possess other classes of binding sites with high and low affinity. Occupancy by exogenous zinc of these additional binding sites inhibits polymerase activity.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Lodz, Poland

    K. Brudzynska, A. Ploszajska, R. Roszczyk & Z. Walter

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  1. K. Brudzynska
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  2. A. Ploszajska
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  3. R. Roszczyk
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  4. Z. Walter
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Brudzynska, K., Ploszajska, A., Roszczyk, R. et al. Internation of zinc ions with DNA-dependent RNA polymerases A, B and C isolated from calf thymus. Mol Biol Rep 8, 77–83 (1982). https://doi.org/10.1007/BF00778508

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  • DOI: https://doi.org/10.1007/BF00778508

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