Abstract
Reassociation of typical single-copy DNAs, like E. coli DNA, even when performed at relatively low temperatures, results in the formation of perfect duplexes with thermal stability very close to that of the native DNA. In contrast, duplexes of mouse repeated DNA as well as duplexes of Streptomyces DNA prepared under the same conditions, show a low thermal stability and undergo post-reassociation changes upon prolonged incubation. These changes, called “maturation” of the DNA duplexes, result in increasing of their thermal stability. Some of the factors affecting the rate of “maturation” are studied. The implication of the “maturation” process in reassociation analysis and in characterization of the heterogeneity of DNA is discussed.
Similar content being viewed by others
References
CowieD.B. and AveryR.J., Carnegie Inst. Year Book69, 528–538 (1971).
CowieD.B. and AveryR.J., Carnegie Inst. Year Book70, 361–365 (1971).
CowieD.B. and PragerL.K., Carnegie Inst. Year Book68, 391–397 (1970).
DoverG.A., Chromosoma53, 153–173 (1975).
IvanovI.G., VenkovP., and MarkovG.G., Preparative Biochem.5, 219–228 (1975).
KohneD.E., ChisconJ.A., and HoyerB.H., Carnegie Inst. Year Book69, 488–501 (1971).
McConaughyB.L., and McCarthyB.J., Biochem. Genet.4, 425–446 (1970).
RiceN., Carnegie Inst. Year Book69, 472–479 (1971).
RiceN., Carnegie Inst. Year Book70, 366–369 (1971).
RiceN., Carnegie Inst. Year Book73, 1098–1102 (1974).
RiceN. and EspositoP., Carnegie Inst. Year Book72, 197–204 (1973).
SteinD.B. and ThompsonW.F., Science189, 888–890 (1975).
SuttonW.D. and McCallumM., J. Mol. Biol.71, 633–656 (1972).
LairdC.D., McConaughyB.L., and McCarthyB.J., Nature224, 149–154 (1969).
UllmannJ.S. and McCarthyB.J., Biochim. Biophys. Acta294, 405–415 (1973).
UllmannJ.S. and McCarthyB.J., Biochim. Biophys. Acta294, 416–424 (1973).
BrittenR.J., GrahamD.E., and NeufeldB.R.: in L.Grossman and K.Moldave (eds.), Methods in Enzymology, Academic Press, New York and London, 1974, Vol. XXIX, p. 363–418.
MarkovG.G. and IvanovI.G., Anal. Biochem.59, 555–563 (1974).
AntonovP., IvanovI.G., and MarkovG.G., FEBS Letters 79, 151–154 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ivanov, I., Antonov, P., Markova, N. et al. “Maturation” of DNA duplexes. Mol Biol Rep 4, 67–71 (1978). https://doi.org/10.1007/BF00775962
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00775962