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Composition of hnRNA-associated proteins in rat liver is specifically altered after cycloheximide treatment

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Abstract

Rats were treated with non-lethal doses of α-amanitin or cycloheximide. Nuclei were prepared and the particles carrying heterogeneous nuclear RNA (30–40 S-particles) isolated by density gradient centrifugation. In the case of α-amanitin the yield of particles was reduced to about 45%. Cycloheximide affected the composition of proteins associated with the nuclear RNA. In particular, the concentration of a 110 000 molecular weight protein as determined by sodium dodecylsulfate gel electrophoresis was reduced to 20–30% after 2 h and then increased to 150–180% of the control before it approached the normal level after 30 h. Possible mechanisms underlying these changes are discussed.

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Abbreviations

hnRNA:

heterogeneous nuclear RNA

hnRNP:

ribonucleoprotein which contains hnRNA

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Authors and Affiliations

  1. Max-Planck-Institut fur Biochemie, 8033, Martinsried bei Munchen, Deutschland

    G. Kostka & A. Schweiger

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  1. G. Kostka
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  2. A. Schweiger
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Kostka, G., Schweiger, A. Composition of hnRNA-associated proteins in rat liver is specifically altered after cycloheximide treatment. Mol Biol Rep 6, 57–61 (1980). https://doi.org/10.1007/BF00775756

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  • DOI: https://doi.org/10.1007/BF00775756

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