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R-loop hybridization of collagen DNA: Separation in Cs2SO4 gradients

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Abstract

High molecular weight poly(A)+ RNA (26–35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in Cs2SO4 gradients from the bulk of DNA yielding double stranded DNA enriched for collagen genes.

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References

  1. WhiteR.L. and HognessD.S., Cell 10, 177 (1977).

    Google Scholar 

  2. ThomasM., WhiteR.L. and DavisR.W., Proc. Natl. Acad. Sci. U.S.A. 73, 2294 (1976).

    Google Scholar 

  3. ChowL.T., GelinasR.E., BrokerTh.R. and RobertsR.J., Cell 12, 1 (1977).

    Google Scholar 

  4. EngelJ.D. and DavidsonN., Biochemistry 17, 3883 (1978).

    Google Scholar 

  5. KinniburghA.J., MertzJ.E. and RossJ., Cell 14, 681 (1978).

    Google Scholar 

  6. KabackD.B., AngererL.M. and DavidsonN., Nucleic Acid Research 6, 2499 (1979).

    Google Scholar 

  7. WellauerP.K. and DavidI.B., Cell 10, 193 (1977).

    Google Scholar 

  8. LehmannC., WarnhoffM., KnoechelW., LangeD., BornJ. and TiedemannH., Molec. Biol. Rep. 4, 217 (1978).

    Google Scholar 

  9. BernsK.I. and ThomasC.A.Jr., J. Mol. Biol. 11, 467 (1965).

    Google Scholar 

  10. TiedemannH., BornJ. and TiedemannH., Wilhelm Roux's Archives 171, 160 (1972).

    Google Scholar 

  11. AdamsSh.L., SobelM.E., HowardB.H., OldenK., YamadaK.M., CrombruggheB.de, and PastanI., Proc. Natl. Acad. Sci. U.S.A. 74, 3399 (1977).

    Google Scholar 

  12. Poly(U)-Sepharose 4 B for affinity chromatography of mRNA. Pharmacia report Jan. 1974–1.

  13. NeufangO. and TiedemannH., Hoppe-Seyler'sZ. Physiol. Chem. 356, 1445 (1975).

    Google Scholar 

  14. CommerfordS.L., Biochemistry 10, 1993 (1971).

    Google Scholar 

  15. KnoechelW., Molecular Biol. Rep. 1, 311 (1974).

    Google Scholar 

  16. BreitkreutzD., DeLeonL.D., PagliaL., ZeichnerM., WilczekJ. and SternR., Biochim. Biophys. Acta 517, 349 (1978).

    Google Scholar 

  17. FrischaufA.M., LehrachH., RosnerC. and BoedtkerH., Biochemistry 17, 3243 (1978).

    Google Scholar 

  18. TostoshevP., HaberR. and CrystalR.G., Biochem. Biophys. Res. Comm. 87, 818 (1979).

    Google Scholar 

  19. MillerE.J., Biochemistry 10, 3030 (1971). Fitzek, P., Rexboldt, F., Hopper, K. and Kuhn, K., Europ. J. Biochem. 38, 396 (1973).

    Google Scholar 

  20. RamachandranG.N. and ReddiA., Biochemistry of Collagen, Plenum Publ. Corp., New York (1976).

    Google Scholar 

  21. MillerE.J. and MakukasV.J., Federation Proc. 33, 1197 (1974).

    Google Scholar 

  22. v.d.MarkH., v.d.MarkK. and GaySt., Devel. Biol. 48, 237 (1976).

    Google Scholar 

  23. AdamsonE.D. and AyersS.E., Cell 16, 953 (1979).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Arnimallee 22, 1000, Berlin 33, West-Germany

    J. Born, B. Wittig, St. Wittig & H. Tiedemann

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  1. J. Born
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  2. B. Wittig
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  3. St. Wittig
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  4. H. Tiedemann
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Born, J., Wittig, B., Wittig, S. et al. R-loop hybridization of collagen DNA: Separation in Cs2SO4 gradients. Mol Biol Rep 6, 159–161 (1980). https://doi.org/10.1007/BF00775410

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  • DOI: https://doi.org/10.1007/BF00775410

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