Skip to main content
SpringerLink
Log in

The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. It is shown that the presence of chelating agents and, especially, the application of considerable mechanical forces in the course of isolation may cause damage to the nucleosomal structure of the chromatin. Moreover, sufficiently great mechanical forces are liable to destroy the structure of the chromatin nucleosomal fibres even when they are packed in structures of a higher order of organization of the chromatin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. HewishD.R., BurgoinL.A., Biochem. and Biophys. Res. Commun.,52, 504, (1973).

    Google Scholar 

  2. NollM., ThomasJ.O., KornbergR.D., Science,187, 1203, (1975).

    Google Scholar 

  3. DoeneckeD., Exp. Cell Res.,100, 223, (1976).

    Google Scholar 

  4. WoodcockC.L.F., SaferJ.P., StanchfieldJ.E., Exp. Cell Res.97, 111, (1976).

    Google Scholar 

  5. VengerovYu.Yu., PopenkoV.I., TikhonenkoA.S., Doklady AN SSSR,232, 1442, (1977).

    Google Scholar 

  6. KiryanovG.I., ManamshyanT.A., PolyakovV.Yu., FaisD., ChentsovYu.S., FEBS Letters,67, 323, (1976).

    Google Scholar 

  7. Vengerov Yu.Yu., Popenko V.I., Lang H., Tikhonenko A.S., Biol. Zentralblatt, in press.

  8. VengerovYu.Yu. PopenkoV.I., Nucl. Acids Res.,4, 3017–3027, (1977).

    Google Scholar 

  9. RisH., J. Cell. Biol.,39, 158, (1968).

    Google Scholar 

  10. SteffensonD. in “The Cell Nucleus”, London, Butterworths, 1960, p. 217.

    Google Scholar 

  11. OudetP., Gross-BellardM., ChambonP., Cell,4, 282, (1975).

    Google Scholar 

  12. HubermanJ.A., Ann. Rev. Biochem.,42, 355, (1973).

    Google Scholar 

  13. GeorgievG.P., IlyinYu.V., TikhonenkoA.S., DobbertN.N., AnanyevaL.N., Molekularnaya Biologiya,1, 815, (1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow, USSR

    V. I. Popenko & Yu. Yu. Vengerov

Authors
  1. V. I. Popenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu. Yu. Vengerov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Popenko, V.I., Vengerov, Y.Y. The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin. Mol Biol Rep 4, 45–50 (1978). https://doi.org/10.1007/BF00775180

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00775180

Keywords

Search

Navigation