Summary
Growth ofCandida albicans strain B 311-10 was observed in a minimal synthetic biotin-free medium, using different glucose concentrations, during the first 30 hours of its development at 28 °C. The yeast's growth was observed spectrophotometrically at 675 nm reading its optical density every hour. The minimal medium of Shepherdet al. [12], with glucose (15 g/L) and biotin was modified: this vitamin was eliminated and the concentration of glucose was gradually lowered to 0.5 g/L. At 5 g/L of glucose and without biotin very good growth was obtained. Based on our results during the first 30 hours of growth, biotin has no influence on the yeast's growth. This medium would be useful for the study of the physiology ofC. albicans during the first period of its development.
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Vidotto, V., Pugliese, A. & Gioannini, P. Growth ofCandida albicans in a minimal synthetic medium without biotin. Mycopathologia 100, 7–15 (1987). https://doi.org/10.1007/BF00769562
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DOI: https://doi.org/10.1007/BF00769562