Abstract
To analyze how many D-region-encoded molecules could be detected inH-2 q, we produced a panel of nine monoclonal antibodies from AKR (KkDk) anti-AKR.M (KkDq) immunizations. All of the Dq region antibodies cross-reacted on Dd and/or Ld, and all except one cross-reacted on Db, confirming the previously observed serologic and amino acid sequence homology between theD-region products ofH-2d,H-2b, andH-2q. All of these monoclonal antibodies precipitated 46 000 dalton molecules from both cell-surface-labeled and biosynthetically labeled BIO.AKM spleen cells, indicating that all were reactive with class I-like molecules. Sequential immunoprecipitation analysis with one of these antibodies, 66-3-5, reveals the presence of a previously unidentified class I-like molecule. Tryptic peptide map analysis reveals that this molecule may be the product of a newly describedH-2D q-region gene.
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Chatterjee-Das, S., Lillehoj, E.P., Hernandez, D.M. et al. Analysis of theD-region products ofH-2444using monoclonal antibodies reveals the expression of a new class I-like molecule. Immunogenetics 25, 7–14 (1987). https://doi.org/10.1007/BF00768827
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DOI: https://doi.org/10.1007/BF00768827