Abstract
The effects of uncouplers (DNP, FCCP), oligomycin, and rotenone on the energetics and mitochondrial ultrastructure in lymphocytes have been studied. We confirmed the previous observations done on Ehrlich ascites and cardiomyocyte culture cells that uncouplers and respiratory inhibitors cause the appearance of ringlike and dumbbell-like mitochondria. It is shown that this effect does not correlate with decrease in ATP concentration, changes in oxygen consumption, or condensation of the mitochondrial matrix. FCCP (2 µM) is more effective in the induction of abnormal-form mitochondria than 240 µM DNP, oligomycin, or rotenone. Combined treatment with DNP, oligomycin, and rotenone or with DNP and rotenone produces an effect as strong as 2 µm FCCP. DNP (240 µM) and FCCP (2 µM) have a similar effect on respiration and intracellular ATP, but only the latter induces condensation of the mitochondrial matrix.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bell, P. R., and Muhlethaler, K. (1964).J. Cell. Biol. 20, 235–248.
Brand, M. D., and Felber, S. M. (1984).Biochem. J. 224, 1027–1030.
Buffa, P., Pasquali-Ronchetti, I., and Coppi, G. (1967). Atti del VI Congresso Italiano di Microscopia Elettronica, Siena, 29–31 Ottobre 1967, Padova, pp. 7–11.
Cittadini, A., Bossi, D., Longhi, G., and Terranova, T. (1975).Mol. Cell. Biochem. 8, 49–57.
Endress, A. G., and Sjolund, R. D. (1976).Am. J. Bot. 63, 1213–1224.
Goppelt-Struebe, M., Kyas, U., and Resch, K. (1986).FEBS Lett. 202, 45–48.
Gulyaeva, N. V., Konoshenko, G. I., and Mokhova, E. N. (1985).Biochem. J. 224, 19–32.
Hackenbrock, C. R. (1968).J. Cell Biol. 37, 345–517.
Igbavboa, U., and Pfeiffer, D. R. (1988).J. Biol. Chem. 263, 1405–1412.
Jacqueline, E., and Jacques, P. (1984).Biochem. Biophys. Res. Commun. 125, 719–727.
Konoshenko, G. I., Markova, O. V., and Bakeeva, L. E. (1986). Molecular Mechanisms and Regulation of Energy Metabolism, Abstracts of Reports, Pushchino, p. 83 (in Russian).
Laiho, K. U., and Trump, B. F. (1975).Lab. Invest. 21, 163–182.
Lehninger, A. L. (1962).Physiol. Rev. 42, 467–517.
Leikina, M. I., Polyakova, I. A., and Chentsov, Yu. I. (1982). Third All-Union Conference on Cell Pathology, Abstracts of Reports, Moscow, pp. 148–150 (in Russian).
Markova, O. V., Konoshenko, G. I., Mokhova, E. N., and Tarakanova, A. N. (1989).Biol. Membr. 6, 488–497 (in Russian).
Mokhova, E. N., and Rozovskaya, I. A. (1986).J. Bioenerg. Biomembr. 4, 265–276.
Olson, L. W. (1973).Protoplasma 78, 129–144.
Osafune, T., Mihara, S., Hase, E., and Ohkuro, I. (1975).J. Electron Microsc. 24, 247–252.
Reynolds, E. S. (1963).J. Cell Biol. 17, 208–212.
Rydzynsky, K., and Cieciura, L. (1980).J. Ultrastruct. Res. 70, 118–127.
Skulachev, V. P. (1972).Transformation of Energy in Biomembranes, Nauka Press, Moscow (in Russian).
Smith, R. A., and Ord, M. J. (1983).Int. Rev. Cytol. 83, 63–134.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Markova, O.V., Mokhova, E.N. & Tarakanova, A.N. The abnormal-shaped mitochondria in thymus lymphocytes treated with inhibitors of mitochondrial energetics. J Bioenerg Biomembr 22, 51–59 (1990). https://doi.org/10.1007/BF00762845
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00762845