Abstract
In a batch-refeed continuous process involving a recombinant Chinese hamster ovary cell line, a brief upset was Occasionally observed during which cell growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.
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Abbreviations
- CHO:
-
Chinese hamster ovary
- rhM-CSF:
-
recombinant human macrophage colony stimulating factor
- RIA:
-
radioimmunoassay
- RP-HPLC:
-
reverse phase high performance liquid chromatography
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Drapeau, D., Luan, YT., Popoloski, J.A. et al. Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process. Cytotechnology 15, 103–109 (1994). https://doi.org/10.1007/BF00762384
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DOI: https://doi.org/10.1007/BF00762384