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Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific

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Abstract

In order to determine whether the enhanced specific antibody productivity (q MAb ) of calcium alginate-entrapped hybridoma is cell line-specific, calcium alginate-entrapped hybridomas (4A2 and DB9G8) were cultivated under the condition where we had previously observed significantly enhancedq MAb of calcium alginate-entrapped S3H5/γ2bA2 hybridoma. Unlike S3H5/γ2bA2 hybridoma, neither 4A2 nor DB9G8 hybridomas showed persistently enhancedq MAb when they were entrapped in calcium alginate beads. The enhancedq MAb of entrapped 4A2 and DB9G8 hybridomas, which was 2–3 times higher than theq MAb of free-suspended cells in a control experiment, was observed only during the early stage of the culture. During the early stage of the culture, the viable cell concentration decreased probably due to cell damage during the entrapment process. As cell growth resumed, theq MAb decreased to the similar level ofq MAb of free-suspended cells within 5–7 days. Thus, we conclude that the enhancedq MAb of calcium alginate-entrapped hybridomas is cell line-specific.

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Authors and Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science and Technology, 373-1, Kuseong-Dong, Yuseong-Gu, 305-701, Taejun, Korea

    Gyun Min Lee & Sang Jick Kim

  2. Department of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbor, MI, USA

    Bernhard O. Palsson

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  1. Gyun Min Lee
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  2. Sang Jick Kim
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  3. Bernhard O. Palsson
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Lee, G.M., Kim, S.J. & Palsson, B.O. Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific. Cytotechnology 16, 1–15 (1994). https://doi.org/10.1007/BF00761774

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  • DOI: https://doi.org/10.1007/BF00761774

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