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Determination of the quantity of ATP and ADP in commercial preparations by various methods

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Conclusions

  1. 1.

    When ATP was determined in a sample of the preparation by the enzymatic (hexokinase) method with subsequent determination of ATP according to the decrease in acid-labile phosphorus, by the spectrophotometrical method, the radioenzymatic method, and by paper chromatography, the various data were in agreement with each other. The data obtained by analysis of the ATP preparation according to 7'-hydrolyz-able phosphorus were high in comparison with the data obtained by the other methods.

  2. 2.

    When ADP was determined in a sample of the ADP preparation by three different methods, spectroenzymology, radioenzymology, and paper chromatography, the results obtained were practically identical.

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Authors and Affiliations

  1. V. M. Bekhterev Institute of Psychoneurology, Leningrad

    G. I. Meerov & B. P. Tsarev

Authors
  1. G. I. Meerov
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  2. B. P. Tsarev
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Additional information

The enzymatic determinations of ATP and ADP were conducted by G. I. Meerov, and the data of the Chromatographie analysis of ATP and ADP in the preparations were provided by B. P. Tsarev.

Translated from Khimiko-Farmatsevticheskii Zhurnal, No. 1, pp. 39–41, January, 1969.

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Meerov, G.I., Tsarev, B.P. Determination of the quantity of ATP and ADP in commercial preparations by various methods. Pharm Chem J 3, 36–38 (1969). https://doi.org/10.1007/BF00759773

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  • DOI: https://doi.org/10.1007/BF00759773

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