Abstract
A simple method to analyze media consumption and by-product build-up in high-cell-density perfusion cultures has been developed. This technique makes use of media replenishment strategy which can be used to decouple the two phenomena. Also, the replenished media method can be used to identify limitation by complex and undefined media components such as bovine serum.
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References
Dallili M and Ollis DF (1989) Transient Kinetics of Hybridoma Growth and Monoconal Antibody Production in Serum-Limited Cultures. Biotechnol. Bioeng. 33: 74.
Dyal D, DeMangel, Munier-Jolain K, Miossec S, Gaehel I (1991) Factors Controlling Cell Profileration and Antibody Production in Mouse Hybridoma Cells: Influence of Amino Acid Supply. Bioteechnol. Bioeng. 38: 561.
Glacken MW (1988) Catabolic Control of Mammalian Cell Culture. Bio/Technology 6: 1041.
Low K, Harbour C (1985) Growth Kinetics of Hybridoma Cells. The Effects of Varying Energy Source Concentrations. Develop biol. Standard 60: 73.
McKinney KL, Dilwith R, Belfort G (1991) Manipulation of Heterogeneous Hybridoma Cultures for Overproduction of Monoclonal Antibodies. Bitechnol. Prog.
Miller WM, Wilke CR, Blanch HW (1988) Kinetic Analysis of Hybridoma Growth and Metabolism in Batch and Continuous Culture: Effect of Nutrient Concentrations, Dilution Rate and pH. Biotechnol. Bioeng. 32: 947.
Ozturk SS, Riley MR, Palsson BO (1991) Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism and Antibody Production. Biotechnol. Bioeng. 39: 418.
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Mahadevan, M.D., Klimkowsky, J.A. & Deo, Y.M. Media replenishment: A tool for the analysis of high-cell density perfusion systems. Cytotechnology 14, 89–96 (1994). https://doi.org/10.1007/BF00758173
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DOI: https://doi.org/10.1007/BF00758173