Abstract
Plasma membrane modifications have been widely recognized as crucial factors in cell injury and death. One of these modifications, surface blebbing, has been considered as an injury-marker associated with a series of biochemical and physiological modifications. Our study focused on the different effects of free radical-induced cell damage by quinone menadione (2-methyl-1,4-naphthoquinone) and by hyperthermic shock (45°C) on the erythroleukemic cell line K.562. Different techniques including immunofluorescence, freeze-fracturing, and electron paramagnetic resonance spectroscopy were employed. Menadione induced the formation of surface blebs, accompanied by a rearrangement of the microfilament system and changes in the distribution of plasma membrane proteins. In contrast, heat-shocked cells showed neither blebbing nor important cytoskeletal changes. Finally, the electron paramagnetic resonance results showed an increase in membrane order not specifically related to the type of free radical-induced stress. These cell death features appear to suggest the existence of two different types ofpathways for necrotic cell death: both treatments induce cell injury and eventual death by modifiting plasma membrane integrity and function. However, one involves cytoskeleton-dependent surface blebbing, whereas the other does not.
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Abbreviations
- EPR:
-
electron paramagnetic resonance
- HS:
-
heat shock
- IMPs:
-
intramembrane particles
- MEN:
-
menadione
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Malorni, W., Paradisi, S., Iosi, F. et al. Two different pathways for necrotic cell death induced by free radicals. Cell Biol Toxicol 9, 119–130 (1993). https://doi.org/10.1007/BF00757574
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DOI: https://doi.org/10.1007/BF00757574