, Volume 13, Issue 1, pp 55–60 | Cite as

A rapid preparation of primary cultures of mouse skeletal muscle cells

  • Laurent Metzinger
  • Philippe Poindron
  • Anne-Catherine Passaquin
Technical Report


We describe a rapid and reproducible technique for establishing primary cultures of skeletal muscle cells from mouse origin. This method was aimed at avoiding extensive enzymatic proteolysis which is commonly used for preparation of primary skeletal muscle cultures. It relies on a Stomacher® blender that allows a rapid and regular mechanical dissociation of muscle samples by repeated shocks. Cultures have been compared to those obtained by a modification of the method of Yaffé (1993) based on tryptic dissociation of rat muscle thighs. The time of preparation was reduced to 1 h and 15 min as compared to 4 h with the technique of Yaffé. Both cultures displayed similar morphologies and exhibited comparable myogenesis processes. Cellular yield, rate of myotube formation and myotube numbers were similar. The expression of myogenesis markers were identical as assessed by determination of acetylcholine receptor number, creatine kinase activity and level of myosin light chain.

Key words

primary muscle cell culture mouse myogenesis markers Stomacher blender trypsinization 



Acetylcholine receptor


creatine kinase (EC


Phosphate buffered saline-free of Ca2+ and Mg2+


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Laurent Metzinger
    • 1
  • Philippe Poindron
    • 1
  • Anne-Catherine Passaquin
    • 1
  1. 1.Département d'Immunologie, d'Immunopharmacologie et de Pathologie, Equipe de Biologie de la cellule musculaireUniversité Louis Pasteur (ULP)Illkirch CedexFrance

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