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Decreasedin vitro chemosensitivity of tumour cells in patients suffering from malignant diseases with a poor prognosis

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Abstract

The aim of the study was to assess the predictive value of MTTin vitro assay for evaluation of tumour cell resistance/sensitivity to cytotoxic drugs. We analyzed 105 samples of malignant cells of different origin. The study included patients with a diagnosis of acute and chronic lymphatic leukaemia, acute and chronic myeloid leukaemia, non-Hodgkin lymphoma, carcinoma of the lung, stomach and liver, rhabdomyosarcoma and breast carcinoma. The results demonstrate outstanding chemosensitivity in the majority of childhood acute lymphoblastic leukaemias, medium chemosensitivity of adult haematopoietic malignant diseases and chemoresistance of solid tumour cells. Our preliminary data suggest a good correlation betweenin vitro MTT assay and clinical curability of individual malignant diseases.

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Abbreviations

ALL:

acute lymphoid leukaemia

AML:

acute myeloid leukaemia

CML:

chronic myeloid leukaemia

LCS50 :

50% leukaemic cell survival

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Authors and Affiliations

  1. Institute of Immunology, Faculty of Medicine, Palacký University, Hněvotínská 3, Olomouc, Czech Republic

    Marián Hajdúch, Mária Šafářová & Evžen Weigl

  2. Department of Paediatrics, Faculty of Medicine, Palacký University, Hněvotínská 3, Olomouc, Czech Republic

    Vladimír Mihál & Petr Antálek

  3. Department of Oncology, Faculty of Medicine, Palacký University, Hněvotínská 3, Olomouc, Czech Republic

    Jozef Minařík

  4. Department of Haematology, Faculty of Medicine, Palacký University, Hněvotínská 3, Olomouc, Czech Republic

    Edgar Faber

Authors
  1. Marián Hajdúch
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  2. Vladimír Mihál
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  3. Jozef Minařík
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  4. Edgar Faber
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  5. Mária Šafářová
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  6. Evžen Weigl
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  7. Petr Antálek
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Hajdúch, M., Mihál, V., Minařík, J. et al. Decreasedin vitro chemosensitivity of tumour cells in patients suffering from malignant diseases with a poor prognosis. Cytotechnology 19, 243–245 (1996). https://doi.org/10.1007/BF00744219

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  • DOI: https://doi.org/10.1007/BF00744219

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