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The stimulation of photophosphorylation and ATPase by artificial redox mediators in chromatophores ofRhodopseudomonas capsulata at different redox potentials

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Abstract

(1) Inhibition of cyclic phosphorylation in chromatophores ofRhodopseudomonas capsulata by antimycin A can be fully reversed by artificial redox mediators, provided the ambient redox potential is maintained around 200 mV. The redox mediator need not be a hydrogen carrier in its reduced form, N-methyl-phenazonium methosulfate and N,N,N′,N′-tetramethyl-p-phenylenediamine being equally effective. However, the mediator needs to be lipophilic. Endogenous cyclic phosphorylation is fastest around 130 mV. A shift to 200 mV can also be observed if high concentrations of artificial redox mediator are present in the absence of antimycin A. (2) ATPase activity ofRhodopseudomonas capsulata, in the light as well as in the dark, activated or not activated by inorganic phosphate, can also be stimulated by N-methylphenazonium methosulfate. This stimulation is highest at redox potentials between 60 to 80 mV and is sensitive to antimycin A. In this case N,N,N′,N′-tetramethyl-p-phenylenediamine is much less effective.

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Abbreviations

PES:

N-methyl-phenazonium ethosulfate

PMS:

N-methyl-phenazonium methosulfate

TMPD:

N,N,N′,N′-tetramethyl-p-phenylenediamine

DAD:

diaminodurene (2,3,5,6-tetramethyl-p-phenylenediamine)

Bchl:

bacteriochlorophyll

FCCP:

carbonylcyanide-p-trifluoromethoxy-phenylhydrazone

E h :

redox potential

E m :

midpoint redox potential

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Authors and Affiliations

  1. Institute of Botany, University of Bologna, Bologna, Italy

    Assunta Baccarini-Melandri, B. Andrea Melandri & Günter Hauska

  2. FB Biologie und Vorklinische Medizin, Institut für Botanik, University of Regensburg, GFR

    Assunta Baccarini-Melandri, B. Andrea Melandri & Günter Hauska

Authors
  1. Assunta Baccarini-Melandri
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  2. B. Andrea Melandri
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  3. Günter Hauska
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Baccarini-Melandri, A., Melandri, B.A. & Hauska, G. The stimulation of photophosphorylation and ATPase by artificial redox mediators in chromatophores ofRhodopseudomonas capsulata at different redox potentials. J Bioenerg Biomembr 11, 1–16 (1979). https://doi.org/10.1007/BF00743157

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