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Chromophorylation (in Escherichia coli) of allophycocyanin B subunits from far-red light acclimated Chroococcidiopsis thermalis sp. PCC7203

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Abstract

Cyanobacterial phycobilisomes funnel the harvested light energy to the reaction centers via two terminal emitters, allophycocyanin B and the core–membrane linker. ApcD is the a-subunit of allophycocyanin B responsible for its red-shifted absorbance (?max 665 nm). Far-red photo-acclimated cyanobacteria contain certain allophycocyanins that show even further red-shifted absorbances (?max > 700 nm). We studied the chromophorylation of the three far-red induced ApcD subunits ApcD2, ApcD3 and ApcD4 from Chroococcidiopsis thermalis sp. PCC7203 during the expression in E. coli. The complex behavior emphasizes that a variety of factors contribute to the spectral red-shift. Only ApcD2 bound phycocyanobilin covalently at the canonical position C81, while ApcD3 and ApcD4 gave only traces of stable products. The product of ApcD2 was, however, heterogeneous. The major fraction had a broad absorption around 560 nm and double-peaked fluorescence at 615 and 670 nm. A minor fraction was similar to the product of conventional ApcD, with maximal absorbance around 610 nm and fluorescence around 640 nm. The heterogeneity was lost in C65 and C132 variants; in these variants only the conventional product was formed. With ApcD4, a red-shifted product carrying non-covalently bound phycocyanobilin could be detected in the supernatant after cell lysis. While this chromophore was lost during purification, it could be stabilized by co-assembly with a far-red light-induced ß-subunit, ApcB3.

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Qian-Zhao Xu, Qi-Ying Tang, Jia-Xin Han, Wen-Long Ding, Bao-Qing Zhao, Ming Zhou & Kai-Hong Zhao

  2. Max-Planck-Institute for Chemical Energy Conversion, Stiftstrasse 34-36, D-45470, Mülheim, Germany

    Wolfgang Gärtner

  3. Department Biologie I, Universität München, D-80638, München, Germany

    Hugo Scheer

  4. Institute for Analytical Chemistry, University of Leipzig, Germany

    Wolfgang Gärtner

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  1. Qian-Zhao Xu
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Xu, QZ., Tang, QY., Han, JX. et al. Chromophorylation (in Escherichia coli) of allophycocyanin B subunits from far-red light acclimated Chroococcidiopsis thermalis sp. PCC7203. Photochem Photobiol Sci 16, 1153–1161 (2017). https://doi.org/10.1039/c7pp00066a

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