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Vicinal dithiol in pig heart mitochondrial F1-ATPase related to thermal or ATP-dependent conformational changes

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Abstract

Active F1-ATPase prepared from pig heart mitochondria can react with about 2 mol of DTNB (5,5′-dithiobis-2-nitrobenzoic acid) or CPDS (6,6′-dithiodinicotinic acid). The reactivity of these thiol reagents decreases if ATP is absent or if F1-ATPase has been submitted to thermal treatment that increases the specific activity without eliminating any contaminating protein. Affinity chromatography on a Sepharose-DTNB column has shown that the thermal treatment of F1-ATPase induces a conformational change of the enzyme that completely prevents it from being retained on the column while the normal active enzyme can be specifically bound to the Sepharose-DTNB column.

A comparative study of the thiols of F1-ATPase reacting with CPDS measured by spectrophotometric estimation of the thione released from CPDS and by [14C]CPDS binding to F1-ATPase suggests involvement of a vicinal dithiol in active F1-ATPase. After CPDS reaction, this vicinal dithiol may become an internal disulfide bridge.

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Authors and Affiliations

  1. Laboratoire de Biologie et Technologie de Membranes du CNRS, Université Claude Bernard de Lyon I, 43, Bd du 11 Novembre 1918, 69621, Villeurbanne, France

    C. Godinot, A. Di Pietro, B. Blanchy, F. Penin & D. C. Gautheron

Authors
  1. C. Godinot
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  2. A. Di Pietro
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  3. B. Blanchy
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  4. F. Penin
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  5. D. C. Gautheron
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Additional information

This work is part of the “Doctorat de Spécialité” of A. Di Pietro (Lyon, 1975) and of B. Blanchy (in preparation). It has been partly presented at the Venice Joint Meeting, Venice, October 1976.

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Godinot, C., Di Pietro, A., Blanchy, B. et al. Vicinal dithiol in pig heart mitochondrial F1-ATPase related to thermal or ATP-dependent conformational changes. J Bioenerg Biomembr 9, 255–269 (1977). https://doi.org/10.1007/BF00743155

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  • DOI: https://doi.org/10.1007/BF00743155

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