Abstract
Isolated beef heart cytochromec oxidase was reconstituted in liposomes by the cholate dialysis method with 85% of the binding site for cytochromec oriented to the outside. Trypsin cleaved specifically subunit VIa and half of subunit IV from the reconstituted enzyme. The kinetic properties of the reconstituted enzyme were changed by trypsin treatment if measured by the spectrophotometric assay but not by the polarographic assay. It is concluded that subunit VIa and/or subunit IV participate in the electron transport activity of cytochromec oxidase.
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References
Bisson, R., Jacobs, B., and Capaldi, R. A. (1980).Biochemistry 19, 4173–4178.
Brautigan, D. L., Ferguson-Miller, S., and Margoliash, E. (1977).Methods Enzymol. 53, 128–164.
Capaldi, R. A., Malatesta, F., and Darley-Usmar, V. M. (1983).Biochim. Biophys. Acta 726, 135–148.
Casey, R. P., Chappell, J. B., and Azzi, A. (1979).Biochem. J. 182, 149–156.
Casey, R. P., Ariano, B. H., and Azzi, A. (1982).Eur. J. Biochem. 122, 313–318.
Eytan, G. D., and Broza, R. (1978).J. Biol. Chem. 253, 3196–3202.
Ferguson-Miller, S., Brautigan, D. L., and Margoliash, E. (1976).J. Biol. Chem. 251, 1104–1115.
Ferguson-Miller, S., Brautigan, D. L., and Margoliash, E. (1978).J. Biol. Chem. 253, 149–159.
Hundt, E., and Kadenbach, B. (1977).Hoppe-Seyler's Z. Physiol. Chem. 358, 1309–1314.
Jarausch, J., and Kadenbach, B. (1982).Hoppe-Seyler's Z. Physiol. Chem. 363, 1133–1140.
Jarausch, J., and Kadenbach, B. (1985a).Eur. J. Biochem. 146, 211–217.
Jarausch, J., and Kadenbach, B. (1985b).Eur. J. Biochem. 146, 219–225.
Kadenbach, B. (1983).Angew. Chem. 95, 273–281;Angew. Chem. Int. Ed. Engl. 22, 275–282.
Kadenbach, B., and Merle, P. (1981).FEBS Lett. 135, 1–11.
Kadenbach, B., and Stroh, A. (1984).FEBS Lett. 173, 374–380.
Kadenbach, B., Büge, U., Jarausch, J., and Merle, P. (1981). InVectorial Resactions in Electron and Ion Transport in Mitochondria and Bacteria (Palmieri, F.,et al. eds.), Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 11–23.
Kadenbach, B., Hartmann, R., Glanville, R., and Buse, G. (1982).FEBS Lett. 138, 236–238.
Kadenbach, B., Jarausch, J., Hartmann, R., and Merle, P. (1983a).Anal. Biochem. 129, 517–521.
Kadenbach, B., Ungibauer, M., Jarausch, J., Büge, U., and Kuhn-Nentwig, L. (1983b).Trends Biochem. Sci. 8, 398–400.
Kadenbach, B., Stroh, A., and Kuhn-Nentwig, L. (1984).Eur. Bioenerg. Conf. 3, 59–60.
Kadenbach, B., Stroh, A., Ungibauer, M., Kuhn-Nentwig, L., Büge, U., and Jarausch, J. (1986).Methods Enzymol.,6 in press.
Kagawa, Y., and Racker, E. (1971).J. Biol. Chem. 256, 5477–5487.
Koppenol, W. H., and Margoliash, E. (1982).J. Biol. Chem. 257, 4426–4437.
Kuhn-Nentwig, L., and Kadenbach, B. (1984).FEBS Lett. 172, 189–192.
Kuhn-Nentwig, L., and Kadenbach, B. (1985a).Eur. J. Biochem. 149, 147–158.
Kuhn-Nentwig, L., and Kadenbach, B. (1985b).Eur. J. Biochem., in press.
Ludwig, B., Downer, N. W., and Capaldi, R. A. (1979).Biochemistry 18, 1401–1407.
Malatesta, F., Darley-Usmar, V., de Jong, C., Prochaska, L. J., Bisson, R., Capaldi, R. A., Steffens, G. C. M., and Buse, G. (1983).Biochemistry 22, 4405–4411.
Merle, P., and Kadenbach, B. (1980a).Hoppe-Seyler's Z. Physiol. Chem. 361, 1257–1259.
Merle, P., and Kadenbach, B. (1980b).Eur. J. Biochem. 105, 499–507.
Merle, P., Jarausch, J., Trapp, M., Scherka, R., and Kadenbach, B. (1981).Biochim. Biophys. Acta 669, 222–230.
Smith, L., Davies, H. C., and Nava, E. (1979).Biochemistry 18, 3140–3146.
Yonetani, T. (1967).Methods Enzymol. 10, 332–335.
Zhang, Y.-Z., Georgevich, G., and Capaldi, R. A. (1984).Biochemistry 23, 5616–5621.
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Büge, U., Kadenbach, B. Effect of trypsin on the kinetic properties of reconstituted beef heart cytochromec oxidase. J Bioenerg Biomembr 17, 375–384 (1985). https://doi.org/10.1007/BF00743110
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DOI: https://doi.org/10.1007/BF00743110