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Autoradiographic studies ofDrosophila gonads following the feeding of14C labelled formaldehyde

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Summary

Male and female larvae ofDrosophila melanogaster at 24, 48, 72 and 96 hours of age were placed upon a standard type of food to which14C labelled formaldehyde had been added. They were left to feed for specified periods of time and then removed and autoradiographs were prepared. The technique used demonstrated that formaldehyde enters the male and female gonad with penetration equally in all regions. The differential sensitivity demonstrated by genetic techniques seems, thus, to be a consequence of differential response to the presence of formaldehyde rather than to differential penetration.

It has been pointed out that a positive autoradiograph indicates the presence of14 C atoms but does not tell anything about the compounds in which they are present.

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Authors and Affiliations

  1. Division of Research, City of Hope Medical Center, Duarte, California, USA

    William D. Kaplan

  2. the Department of Infectious Diseases, Medical School, University of Calif., Los Angeles

    William D. Kaplan

  3. Experimental Radiopathology Research Unit, Hammersmith Hospital, London, England

    Stephen R. Pelc

Authors
  1. William D. Kaplan
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  2. Stephen R. Pelc
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Work carried out during term as Fellow, Agricultural Research Council of United Kingdom at Institute of Animal Genetics, Edinburgh/Scotland

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Kaplan, W.D., Pelc, S.R. Autoradiographic studies ofDrosophila gonads following the feeding of14C labelled formaldehyde. Z.Ver-erbungslehre 87, 356–364 (1956). https://doi.org/10.1007/BF00737156

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  • DOI: https://doi.org/10.1007/BF00737156

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