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A putative carbohydrate-binding domain of the lactosebindingCytisus sessilifolius anti-H(O) lectin has a similar amino acid sequence to that of thel-fucose-bindingUlex europaeus anti-H(O) lectin

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Abstract

The complete amino acid sequence of a lactose-bindingCytisus sessilifolius anti-H(O) lectin II (CSA-II) was determined using a protein sequencer. After digestion of CSA-II with endoproteinase Lys-C or Asp-N, the resulting peptides were purified by reversed-phase high performance liquid chromatography (HPLC) and then subjected to sequence analysis. Comparison of the complete amino acid sequence of CSA-II with the sequences of other leguminous seed lectins revealed regions of extensive homology. The amino acid sequence of a putative carbohydrate-binding domain of CSA-II was found to be similar to those of several anti-H(O) leguminous lectins, especially to that of thel-fucose-bindingUlex europaeus lectin I (UEA-I).

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Abbreviations

BPA:

Bauhinia purpurea lectin

Con A:

concanavalin A

CMA-I:

Cytisus multiflorus lectin I

CMA-II:

Cytisus multiflorus lectin II

CSA-I:

Cytisus sessilifolius lectin I

CSA-II:

Cytisus sessilifolius lectin II

CSII:

Cytisus scoparius lectin II

ECorL:

Erythrina corallodendron lectin

GSIV:

Griffonia simplicifolia lectin IV

HPLC:

high performance liquid chromatography

LAA-I:

Laburnum alpinum lectin I

LAA-II:

Laburnum alpinum lectin II

LOL:

Lathyrus ochrus lectin

LTA:

Lotus tetragonolobus lectin

MAH:

Maackia amurensis haemagglutinin

PSA:

Pisum sativum lectin

SDS:

sodium dodecyl sulfate

TFA:

trifluoroacetic acid

UEA-I:

Ulex europaeus lectin I

UEA-II:

Ulex europaeus lectin II

VFA:

Vicia faba lectin

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Author notes

  1. Toshiaki Osawa

    Present address: Yakult Central Institute for Microbiological Research, Yaho, Kunitachi-shi, Tokyo

Authors and Affiliations

  1. Department of Cancer Biology and Molecular Immunology (formerly Division of Chemical Toxicology and Immunochemistry), Faculty of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Yukiko Konami, Kazuo Yamamoto, Toshiaki Osawa & Tatsuro Irimura

Authors
  1. Yukiko Konami
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  2. Kazuo Yamamoto
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  3. Toshiaki Osawa
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  4. Tatsuro Irimura
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Konami, Y., Yamamoto, K., Osawa, T. et al. A putative carbohydrate-binding domain of the lactosebindingCytisus sessilifolius anti-H(O) lectin has a similar amino acid sequence to that of thel-fucose-bindingUlex europaeus anti-H(O) lectin. Glycoconjugate J 12, 128–134 (1995). https://doi.org/10.1007/BF00731356

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  • DOI: https://doi.org/10.1007/BF00731356

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