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Ganglioside alterations in YAC-1 cells cultivated in serum-supplemented and serum-free growth medium

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Abstract

Gangliosides of the ‘GM1b-pathway’ (GM1b and GalNAc-GM1b) have been found to be highly expressed by the mouse T lymphoma YAC-1 grown in serum-supplemented medium, whereas GM2 and GM1 (‘GM1a-pathway’) occurred only in low amounts [Müthing, J., Peter-Katalinić, J., Hanisch, F.-G., Neumann, U. (1991)Glycoconjugate J 8:414–23]. Considerable differences in the ganglioside composition of YAC-1 cells grown in serum-supplemented and in well defined serum-free medium were observed. After transfer of the cells from serum-supplemented medium (RPMI 1640 with 10% fetal calf serum) to serum-free medium (RPMI 1640 with well defined supplements), GM1b and GalNAc-GM1b decreased and only low amounts of these gangliosides could be detected in serum-free growing cells. The expression of GM1a was also diminished but not as strongly as that of GM1b and GalNAc-GM1b. These growth medium mediated ganglioside alterations were reversible, and the original ganglioside expression was achieved by readaptation of serum-free growing cells to the initial serum-supplemented medium. On the other hand, a ‘new’ ganglioside, supposed to represent GalNAc-GD1a and not expressed by serum-supplemented growing cells, was induced during serum-free cultivation, and increased strongly after readaptation. These observations reveal that the ganglioside composition ofin vitro cultivated cells can be modified by the extracellular environment due to different supplementation of the basal growth medium.

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Authors and Affiliations

  1. Institut für Zellkulturtechnik, Technische Fakultät, Universität Bielefeld, Bielefeld, Germany

    Johannes Müthing & Antje Pörtner

  2. Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany

    Volker Jäger

Authors
  1. Johannes Müthing
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  2. Antje Pörtner
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  3. Volker Jäger
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Additional information

Abbreviations: BSA, bovine serum albumin GSL(s), glycosphingolipid(s); HPTLC, high-performance thin-layer chromatography; LDL, low density lipoprotein; NeuAc,N-acetylneuraminic acid; NeuGc,N-glycoloylneuraminic acid. The designation of the following glycosphingolipids follows IUPAC-IUB recommendations. GgOse3Cer or gangliotriaosylceramide, GalNAcβ1-4Galβ1-4GlcCer; GgOse4Cer or gangliotetraosylceramide, Galβ1-3GalNAcβ1-4Glaβ1-4GlcCer; GgOse5Cer or gangliopentaosylceramide, GalNAcβ1-4Galβ1-3GalNAcβ1-4Galβ1-4GlcCer; GgOse6Cer or gangliohexaosylceramide, Galβ1-3GalNAcβ1-4Galβ1-3GalNAcβ1-4Galβ1-4GlcCer or GgOse6Cer; II3NeuAc-GgOse3Cer or GM2; II3NeuAc-GgOse4Cer or GM1 or GM1a; IV3NeuAc-GgOse4Cer or GM1b; IV3NeuAc-GgOse5Cer or GalNAc-GM1b; IV3NeuAc-GgOse6Cer or Gal-GalNAc-GM1b; IV3NeuAc, II3NeuAc-GgOse4Cer or GD1a; II3(NeuAc)2-GgOse4Cer or GD1b; IV3NeuAc, III6NeuAc-GgOse4Cer or GD1a; IV3NeuAc, II3NeuAc-GgOse5Cer or GalNAc-GD1a.

Enzymes: Vibrio cholerae andArthrobacter ureafaciens neuraminidase (EC 3.2.1.18).

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Müthing, J., Pörtner, A. & Jäger, V. Ganglioside alterations in YAC-1 cells cultivated in serum-supplemented and serum-free growth medium. Glycoconjugate J 9, 265–273 (1992). https://doi.org/10.1007/BF00731138

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  • DOI: https://doi.org/10.1007/BF00731138

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