Abstract
A near full-length cDNA (1.9 kb) was used as probe forin situ hybridization to assign one of the two highly homologous 4-coumarate: CoA ligase genes in parsley (Petroselinum crispum) to the short arm of a submetacentric chromosome. The results suggest, but do not definitely prove, that the second gene is located on a metacentric chromosome and is thus unlinked from the other.
Similar content being viewed by others
Abbreviations
- CHS:
-
chalcone synthase
- 4CL:
-
4-coumarate:CoA ligase
- Kb:
-
kilobases (kilobasepairs)
References
Ambros PF, Matzke MA, Matzke AJM (1986) Chromosoma (Berl.) 94: 11–18
Bianchi N, Lima-De-Faria A, Jaworska H (1964) Hereditas 51: 207–211
Camargo M, Cervenka J (1982) Am. J. Hum. Genet. 34: 757–780
Chandler ME, Yunis JJ, (1978) Cytogenet. Cell Genet. 22: 352–356
Chappell J, Hahlbrock K (1984) Nature 311: 76–78
Douglas C, Hoffmann H, Schulz W, Hahlbrock K (1987) EMBO J. 6: 1189–1195
Feinberg AP, Vogelstein B (1983) Anal. Biochem. 132: 6–13
Gerhard DS, Kawasaki ES, Bancroft FC, Szabo P (1981) Proc. Natl. Acad. Sci. USA 78: 3755–3759
Hahlbrock K, Grisebach H (1979) Annu. Rev. Plant Physiol. 30: 105–130
Henderson AS (1982) Int. Rev. Cytol. 76: 1–46
Herrmann A, Schulz W, Hahlbrock K (1988) Mol. Gen. Genet. 212: 93–98
Huang PL, Hahlbrock K, Somssich IE (1988) Mol. Gen. Genet. 211: 143–147
Jhanwar SC, Neel BG, Haywart WS, Changanti RSK (1983) Proc. Natl. Acad. Sci. USA 80: 4794–4797
Kao FT (1985) International Review of Cytology 96: 51–88
Kombrink E, Hahlbrock K (1986) Plant Physiol. 81: 216–221
Lozoya E, Hoffmann H, Douglas C, Schulz W, Scheel D, Hahlbrock K (1988) Eur. J. Biochem. 176: 661–667
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York
Mouras A, Saul MW, Essad S, Potrykus I (1987) Mol. Gen. Genet. 207: 204–209
Shen D-L, Wang Z-F, Wu M (1987) Chromosoma (Berl.) 95: 311–314
Author information
Authors and Affiliations
Additional information
Communicated by I. Potrykus
Rights and permissions
About this article
Cite this article
Huang, PL., Hahlbrock, K. & Somssich, I.E. Chromosomal localization of parsley 4-coumarate: CoA ligase genes by in situ hybridization with a complementary DNA. Plant Cell Reports 8, 59–62 (1989). https://doi.org/10.1007/BF00716838
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00716838