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Energy-requiring uptake of protoberberine alkaloids by cultured cells ofThalictrum flavum

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Abstract

Cultured cells ofThalictrum flavum take up berberine exogenously added to medium against the concentration gradient. This uptake was temperature-dependent and sensitive to plasma membrane-bound ATPase inhibitors such as sodium orthovanadate and diethylstilbestrol, indicating that the process is mediated by an energy-requiring system. The time-course of pH-shift during berberine uptake suggests the participation of a berberine-proton antiport system in the berberine uptake by the cultured cells. In addition, the existence of a specific transport system was suggested by the competitive inhibition of berberine uptake by berberine analogues, coptisine and jatrorrhizine.

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Author notes

  1. H. Yamamoto

    Present address: Gifu Pharmaceutical University, Mitahora-higashi 5-6-1, 502, Gifu, Japan

  2. M. Suzuki

    Present address: Bioscience Research Center, Mitsui Petrochemical Industries Ltd., Waki-cho, Kuga-gun, 740, Yamaguchi, Japan

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyoto University, 606, Kyoto, Japan

    H. Yamamoto, M. Suzuki, T. Kitamura, H. Fukui & M. Tabata

Authors
  1. H. Yamamoto
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  2. M. Suzuki
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  3. T. Kitamura
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  4. H. Fukui
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  5. M. Tabata
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Additional information

Communicated by F. Constabel

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Yamamoto, H., Suzuki, M., Kitamura, T. et al. Energy-requiring uptake of protoberberine alkaloids by cultured cells ofThalictrum flavum . Plant Cell Reports 8, 361–364 (1989). https://doi.org/10.1007/BF00716674

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  • DOI: https://doi.org/10.1007/BF00716674

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