Skip to main content
SpringerLink
Log in

Regulation of cell proliferation and morphogenesis by amino acids inBrassica tissue cultures and its correlation with threonine deaminase

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The effect of amino acids has been investigated with respect to the capacity ofBrassica cultures to undergo proliferation and differentiation. Hormone medium without any amino acid resulted in 6% shoot formation. Addition of optimal concentrations of L-leucine and L-isoleucine enhanced shoot formation upto 30% and 60%, respectively. L-methionine, L-threonine and pyruvic acid supported only proliferation but no differentiation. Amino acids had a marked effect on the activity of enzyme threonine deaminase (TD), bothin vivo andin vitro. TD in proliferating callus cultures was 3-fold higher than in differentiating cultures. Amino acids which induced cell proliferation increased TD while those which supported differentiation repressed it. Amino acids which did not alter TD activity had no effect on morphogenesis. The results suggest that amino acids play a regulatory role inBrassica morphogenesis which can be correlated with the activity of threonine deaminase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Basu A, Sethi U, Guha-Mukherjee S (1988) Plant Science 58:25–33.

    Google Scholar 

  • Bradford MM (1976) Anal. Biochem. 72:249–254.

    Google Scholar 

  • Dougall DK (1970) Phytochemistry 9:959–964.

    Google Scholar 

  • Fukunaga Y, King J (1982) Plant Sci. Lett. 24:45–54.

    Google Scholar 

  • Gamborg OL, Miller RD, Ojima K (1968) Exp Cell Res. 50:151–158.

    Google Scholar 

  • Giovanelli J, Mudd SH, Datko AH (1988) Plant Physiol. 86:369–377.

    Google Scholar 

  • Holmberg S, Peterson JGL (1988) Curr Genet. 13:207–217.

    Google Scholar 

  • Kirchner SC, Bryan JK (1985) Plant Physiol. 77:S-109.

  • Miflin BJ, Cave PR (1972) J. Exp. Bot. 23: 511–516.

    Google Scholar 

  • Modi SR, Mazumdar R (1966) Indian J. Biochem. 3:215–218.

    Google Scholar 

  • Ronchi VN, Caligo MA, Nozzolini M, Luccarini G (1984) Plant Cell Reports 3:210–214.

    Google Scholar 

  • Sethi U, Basu A, Guha-Mukherjee S (1988) Plant Science 56:167–175.

    Google Scholar 

  • Tupy J, Hrabetova E, Capkova V (1983) Plant Sci. Lett. 30:91–98.

    Google Scholar 

  • Umbarger HE (1973) Advances in Enzymology 37:344–395.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110 067, New Delhi, India

    Atanu Basu, Urmil Sethi & Sipra Guha-Mukherjee

Authors
  1. Atanu Basu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Urmil Sethi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sipra Guha-Mukherjee
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I.K. Vasil

Rights and permissions

Reprints and permissions

About this article

Cite this article

Basu, A., Sethi, U. & Guha-Mukherjee, S. Regulation of cell proliferation and morphogenesis by amino acids inBrassica tissue cultures and its correlation with threonine deaminase. Plant Cell Reports 8, 333–335 (1989). https://doi.org/10.1007/BF00716667

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00716667

Keywords

Search

Navigation