Abstract
Cell suspensions were established from 12 rice genotypes including Indica type, Japonica type and cytoplasmic male sterile (cms) rice lines of both types. 133 green plants were regenerated from protoplasts isolated from an Indica type rice and 15 plants were obtained from a Japonica type cms rice. A cell suspension, from which protoplasts can be isolated (about 106/ml) but from which cell division has not yet been induced, has been established from the most widely used Indica type cms rice which contains Wild Abortive (WA) cytoplasm.
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Abdullah R, Cocking EC, Thompson JA (1986) Bio/Technology 4:1087–1090
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci Sin 18:659–668
Constabel F (1975) In: Gamborg OL, Wetter LR (eds) Plant Tissue Culture Methods, NRCC, Saskatoon, pp 11–16
Coulibaly MY, Demarly Y (1986) Z Pflanzenzuchtg 96:79–81
Fujimura T, Sakurai M, Akage H, Negishi T, Hirose A (1985) Plant Tissue Culture Lett 204:204–207
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Bio/Technology 6:1072–1074
Vasil V, Vasil IK (1984) In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Academic Press, pp. 398–404
Yamada Y, Yang ZQ, Tang DT (1986) Plant Cell Reports 5:85–88
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Communicated by G.C. Phillips
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Wang, D., Miller, P.D. & Söndahl, M.R. Plant regeneration from protoplasts of Indica type rice and CMS rice. Plant Cell Reports 8, 329–332 (1989). https://doi.org/10.1007/BF00716666
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DOI: https://doi.org/10.1007/BF00716666