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Measurement of the translational diffusion constant of G-actin by photon correlation spectroscopy

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Summary

Photon correlation spectroscopic measurements on monomeric actin have yielded a translational diffusion constant of 8.13×10−7 cm2 s−1 after correction for the contribution of the back-reflections of the main beam. This value corresponds to a sphere with a radius of 2.6 nm and 50% hydration, or to a prolate ellipsoid with axes 2.0 and 4.0 nm and 30% hydration.

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Author notes

  1. Kee Woo Rhee

    Present address: Department of Chemistry, Syracuse University, 13210, Syracuse, New York, USA

Authors and Affiliations

  1. The Thomas C. Jenkins Department of Biophysics, The Johns Hopkins University, 21218, Baltimore, Maryland, USA

    Charles Montague, Kee Woo Rhee & Francis D. Carlson

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  1. Charles Montague
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  2. Kee Woo Rhee
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  3. Francis D. Carlson
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Montague, C., Rhee, K.W. & Carlson, F.D. Measurement of the translational diffusion constant of G-actin by photon correlation spectroscopy. J Muscle Res Cell Motil 4, 95–101 (1983). https://doi.org/10.1007/BF00711960

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  • DOI: https://doi.org/10.1007/BF00711960

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