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Characterization of myosin heavy chain by cyanogen bromide peptide maps

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Summary

Procedures have been developed for the preparation of pure myosin heavy chain (h-myosin) by preparative gel electrophoresis, and for the characterization of h-myosin by cyanogen bromide peptide mapping. Major sources of error are the oxidation of methionine and the proteolytic splitting of the chain during purification. These errors have been eliminated. A peculiar feature is the doubling or quadrupling of a peptide of molecular weight 17 000. The results show structural differences between isomyosins derived from myonal types within the same animal, as well as interspecies differences.

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Authors and Affiliations

  1. Department of Physiology and Heart Association Cardiovascular Research Laboratory, University of California, 90024, Los Angeles, U.S.A.

    W. F. H. M. Mommaerts, Klara Vegh, Krikor Seraydarian, Kay Meier & Dan Rittschof

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  1. W. F. H. M. Mommaerts
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  2. Klara Vegh
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  3. Krikor Seraydarian
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  4. Kay Meier
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  5. Dan Rittschof
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Mommaerts, W.F.H.M., Vegh, K., Seraydarian, K. et al. Characterization of myosin heavy chain by cyanogen bromide peptide maps. J Muscle Res Cell Motil 3, 129–144 (1982). https://doi.org/10.1007/BF00711938

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  • DOI: https://doi.org/10.1007/BF00711938

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