Summary
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1.
The effect of interleukin-1 (IL-1) was studied on voltage-activated ion currents of the identified central neurons ofHelix pomatia L. using a twomicroelectrode voltage clamp. The voltage-activated inward current (I Ca) was decreased, whereas the outward current (I net K) was increased by IL-1.
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2.
IL-1 affects both the transient and the delayed rectifying potassium currents. The IL-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. The threshold concentration for IL-1 was 2 U/ml.
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The proposed modulatory effect of IL-1 appears to have more than one site of action on the neuron membrane ion channels.
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4.
Rabbit anti-human IL-1 polyclonal antiserum eliminated the IL-1 effects on the voltage-activated inward and outward currents. This is the first report demonstrating a direct effect of IL-1 modulation of voltage-activated ion currents on neurons of mollusks.
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Szûcs, A., Stefano, G.B., Hughes, T.K. et al. Modulation of voltage-activated ion currents on identified neurons ofHelix pomatia L. by interleukin-1. Cell Mol Neurobiol 12, 429–438 (1992). https://doi.org/10.1007/BF00711543
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DOI: https://doi.org/10.1007/BF00711543