Summary
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1.
The possibility that a long-lasting neuronal activation regulates the expression of muscarinic cholinergic receptors was studied with three cultured neuronal cell lines.
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2.
Continuous depolarization of a subclone of the neuroblastoma-glioma NG108-15 hybrid cells with potassium chloride increased by 45–75% the number of cholinergic muscarinic receptors, monitored with3H-QNB, whereas a short incubation with KC1 for 10 min or 6 hr had no effect.
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3.
The calcium channel blocker verapamil increased the effect of KC1.
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4.
Two cell lines, named SC9 and WC5, that originate from the rat brain, also bind3H-QNB. They were therefore used to test whether the effect of chronic depolarization is universal. Depolarized SC9 and WC5 cells, in the presence or absence of verapamil, did not show an increased3H-QNB binding.
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5.
Muscarinic receptors of both SC9 and WC5 cells have a higher affinity to pirenzepine than the M-3 receptor subtype of the neuroblastoma-glioma cells, suggesting therefore that the two rat brain cell lines possess M-1 or M-2 receptors.
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6.
The physiological significance of this differential role of depolarization on the expression of different muscarinic receptors is discussed in the context of their postreceptor second messengers.
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References
Alzheimer, A. (1907). Uber ein eigenartige erkrankung der hirnrinde.Allg. Z. Psychiat. 64146–148.
Birdsall, N. J. M., and Hulme, E. C. (1983). Muscarinic receptor subclasses.Trends Pharmacol. Sci. 4459–463.
Blaustein, M. P. (1975). Effects of potassium, veratridine and scorpion venom on calcium accumulation and transmitter release by nerve terminals in vitro.J. Physiol. 247617–655.
Brown, S. L., and Brown, J. H. (1983). Muscarinic stimulation of phosphatidylinositol metabolism in atria.Mol. Pharmacol. 24351–356.
Cohen, S., and Sokolovsky, M. (1987). Complexity apparent in muscarinic mechanisms.Trend Pharmacol. Sci. 841–44.
Collerton, D. (1986). Cholinergic function and intellectual decline in Alzheimer's disease.Neuroscience 191–28.
Decker, M. W. (1987). The effect of aging on hippocampal and cortical projections of the forebrain cholinergic system.Brain Res. Rev. 12423–438.
Deutsch, J. A. (1971). The cholinergic synapse and the site of memory.Science 174788–794.
Drachman, D. A., and Leavitt, J. (1974). Human memory and the cholinergic system: A relationship to aging?Arch. Neurol. 30113–121.
Evans, T., Smith, M. M., Tanner, L. I., and Harden, T. K. (1984). Muscarinic cholinergic receptors of two cell lines that regulate cyclic AMP metabolism by different molecular mechanisms.26:395–404.
Fambrough, D. M., Devreotes, P. N., Gardner, J. M., and Card, D. J. (1979). The life history of acetylcholine receptors.Prog. Brain Res. 49325–334.
Frank, E. (1987). The influence of neuronal activity on patterns of synaptic connections.Trends Neurosci. 10188–190.
Giotta, G. J., and Cohn, M. (1981). The expression of glial fibrillary acidic protein in a rat cerebellar cell line.J. Cell. Physiol. 107219–230.
Giotta, G. J., Heitzmann, J., and Cohn, M. (1980). Properties of two temperature-sensitive Rous sarcoma virus transformed cerebellar cell lines.Brain Res. 202445–458.
Giotta, G. J., Heitzmann, J., and Cohn, M. (1982). Immunological identification of cerebellar cell lines.Dev. Brain Res. 4209–221.
Goddard, G. A., and Robinson, J. D. (1976). Uptake and release of calcium by rat brain synaptosomes.Brain Res. 110331–350.
Hammer, R., Berrie, C. P., Birdsall, N. J. M., Burgen, A. S. V., and Hulme, E. C. (1980). Pirenzepine distinguishes between different subclasses of muscarinic receptors.Nature 28390–92.
Hamprecht, B. (1977). Structural, electrophysiological, biochemical and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture.Int. Rev. Cytol. 4999–170.
Iversen, L. L. (1986). The cholinergic hypothesis of dementia.Trends Pharmacol. Sci. 744–45.
Klee, W. A., and Nirenberg, M. (1974). A neuroblastoma × glioma hybrid cell line with morphine receptors.Proc. Natl. Acad. Sci. USA 713474–3477.
Liles, W. C., and Nathanson, N. M. (1987). Regulation of muscarinic acetylcholine receptor number in cultured neuronal cells by chronic membrane depolarization.J. Neurosci. 72556–2563.
Mattera, R., Pitts, B. J. R., Entman, M. L., and Birnbaumer, L. (1985). Guanine regulation of a mammalian myocardial muscarinic receptor system.J. Biol. Chem. 2607410–7421.
Mckinney, M., Stenstrom, S., and Richelson, E. (1985). Muscarinic receptors and binding in a murine neuroblastoma clone NIE-115.Mol. Pharmacol. 27223–235.
Murphy, S., Pearce, B., and Morrow, C. (1986). Astrocytes have both M1 and M2 muscarinic receptor subtypes.Brain Res. 364177–180.
Peralta, E. G., Ashkenazi, A., Winslow, J. W., Smith, D. H., Ramachandran, J., and Capon, D. J. (1987). Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.Embo. J. 63923–3929.
Perry, E. K., Tomlinson, B. E., Blessed, G., Bergmann, K., Gibson, P. H. and Perry, R. H. (1978). Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia.Br. Med. J. 21457–1459.
Rascol, O., and Lamour, Y. (1987). Effect of M-1 and M-2 muscarinic agonists on septo-hippocampal and other central neurons in the rat.Soc. Neurosci. Abstr. 13484.
Schwarcz, R. D., Coughenour, L. L., Davis, R. E., Moos, W. H., and Spencer, C. J. (1987). Efficacy at central muscarinic receptors: Correlation of receptor binding with phosphatidylinositol turnover,3H-acetylcholine release, andin vivo behavioral activity.Soc. Neurosci. Abstr. 13487.
Simantov, R., and Levy, R. (1984). Opiate receptor mechanisms: Characterization of selected neuroblastoma-glioma cells with an enhanced response to opiate antagonists. InCentral and Peripheral Endorphins: Basic and Clinical Aspects (E. E. Müller and A. R. Genazzani, Eds.), Raven Press, New York, pp. 65–70.
Simantov, R., Levy, R., and Baram, D. (1982). Down regulation of enkephalin (δ) receptors: Demonstration in membrane-bound and solubilized receptors.Biochim. Biophys. Acta 721478–484.
Supavilai, P., Bobirnac, I., and Karobath, M. (1987). Characterization of muscarinic autoreceptors in the rat hippocampus and frontal cortex.Soc. Neurosci. Abstr. 13485.
Tanner, L. I., Harden, T. K., Wells, J. N., and Martin, M. W. (1986). Identification of the phosphodiesterase regulated by muscarinic cholinergic receptors of 1321N1 human astrocytoma cells.Mol. Pharmacol. 29455–460.
Walicke, P. A., Campenot, R. B., and Patterson, P. H. (1977). Determination of transmitter function by neuronal activity.Proc. Natl. Acad. Sci. USA 745767–5771.
Yamamura, H. I., and Snyder, S. H. (1974). Muscarinic cholinergic binding in rat brain.Proc. Natl. Acad. Sci. USA 711725–1729.
Zigmond, R. E., and Bowers, C. W. (1981). Influence of nerve activity on the macromolecular content of neurons and their effector organs.Annu. Rev. Physiol. 43673–687.
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Simantov, R., Levy, R. Neuronal membrane depolarization and the control of cholinergic muscarinic receptors: selective effect on different neuronal cell types. Cell Mol Neurobiol 9, 87–94 (1989). https://doi.org/10.1007/BF00711445
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DOI: https://doi.org/10.1007/BF00711445